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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Gamma radiation effects on alpha-lactalbumin: structural modifications.
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Gamma radiation effects on alpha-lactalbumin: structural modifications.

机译:伽玛射线对α-乳白蛋白的影响:结构修饰。

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摘要

Alpha-lactalbumin was irradiated in the lyophilized state in air at ambient temperature. The irradiated protein was examined by size exclusion chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis, circular dichroism, and microcalorimetry. Irradiation induced the loss of aromatic amino acids and of helicity so that fragmentation and aggregation products were obtained. The thermodynamic properties of the protein were also modified. The irradiated protein had lower stability, however, the temperature at which denaturation occurred process remained constant.
机译:在环境温度下在空气中以冻干状态照射α-乳清蛋白。通过尺寸排阻色谱,十二烷基硫酸钠聚丙烯酰胺凝胶电泳,圆二色性和微量量热法检查照射的蛋白质。辐照引起芳香族氨基酸和螺旋度的损失,从而获得碎片和聚集产物。蛋白质的热力学性质也被修饰。辐照的蛋白质具有较低的稳定性,但是变性过程发生的温度保持恒定。

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