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首页> 外文期刊>Fisheries and Aquatic Sciences >Molecular Characterization of Cytoskeletal Beta-Actin and its Promoter in the Javanese Ricefish Oryzias javanicus
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Molecular Characterization of Cytoskeletal Beta-Actin and its Promoter in the Javanese Ricefish Oryzias javanicus

机译：爪哇稻鱼Oryzias javanicus中细胞骨架β-肌动蛋白及其启动子的分子表征

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摘要

We characterized the cytoskeletal beta-actin (beta-ACT) gene (actb) and its 5'-upstream regulatory region in the Javanese ricefish Oryzias javanicus. The gene and protein structures were deduced from amino acid sequences of the actb gene and conservedin the teleost lineage. The O. javanicus actb gene has common transcription factor binding motifs in its regulatory region found in tele-ostean orthologues. Following quantitative reverse transcription-PCR, actb gene transcripts were detected in all tissues examined' however, the basal expression levels were different. During early development, O. javanicus actb mRNA levels showed a gradual increase and peaked between late somitogenesis and the heartbeat stage. Microinjection of O. javanicus embryos with the actb gene promoter-driven red fluorescent protein (RFP) gene reporter vector showed a ubiquitous distribution of RFP signals, although most exhibited a mosaic pattern of transgene expression. A small number of microinjected embryos displayed a wide distribution of RFP signals over their entire body, which resembled the expression pattern of endogenous actb. Data from this study provide a basis to develop a transgenic system with ubiquitous expression of foreign genes in O. javanicus.
机译：我们表征了爪哇稻鱼Oryzias javanicus中的细胞骨架β-肌动蛋白（beta-ACT）基因（actb）及其5'-上游调节区。该基因和蛋白质结构是由actb基因的氨基酸序列推导而来的，并保留了硬骨鱼谱系。爪哇O. javanicus actb基因在远洋直系同源物中发现其调节区域具有共同的转录因子结合基序。在定量逆转录-PCR之后，在所有检查的组织中检测到actb基因转录物，但是基础表达水平是不同的。在早期发育过程中，爪哇O. javanicus actb mRNA的水平逐渐升高，并在晚期发生和心跳期之间达到峰值。用actb基因启动子驱动的红色荧光蛋白（RFP）基因报告基因载体对爪哇稻的显微注射显示RFP信号无处不在，尽管大多数表现出转基因表达的镶嵌模式。少量显微注射的胚胎在其整个体内显示出广泛的RFP信号分布，这类似于内源性actb的表达模式。这项研究的数据为开发一种在爪哇稻中广泛表达外源基因的转基因系统提供了基础。

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