Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography

Seung Hwan Hwang; Jai Man Jang; Soon Sung Lim

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Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography

机译：高速逆流色谱法分离西番莲中的富马甾醇

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We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powderedP. sil-iquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative light-scattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol

机译：我们在这里报告了使用高速逆流色谱法（HSCCC）制备和分离来自Pelvetia siliquosa的生物活性成分岩藻甾醇的情况。通过超声提取粉状P获得粗提取物。使用二氯甲烷，然后通过HSCCC进行分离和纯化，并结合蒸发光散射检测。使用正庚烷：甲醇两相溶剂系统成功进行了制备性HSCCC

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《Fisheries and Aquatic Sciences》 |2012年第3期|共5页
作者
Seung Hwan Hwang; Jai Man Jang; Soon Sung Lim;
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正文语种 eng
中图分类水产、渔业;
关键词
Pelvetia siliquosa; Fucosterol; High-speed countercurrent chromatography; Evaporative light-scattering detection;

机译：四川梧桐;岩藻甾醇;高速逆流色谱;蒸发光散射检测;

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专利

1. Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography [J] . Seung Hwan Hwang, Jai Man Jang, Soon Sung Lim Fisheries and Aquatic Sciences . 2012,第3期

机译：高速逆流色谱法分离西番莲中的富马甾醇
2. Determination of Fucosterol in the Marine Algae Pelvetia siliquosa by Gas Chromatography [J] . Sanghyun Lee, Yeon Sil Lee, Sang Hoon Jung, Natural product sciences . 2004,第1期

机译：气相色谱法测定海洋藻类中Pelvetia siliquosa中的岩藻甾醇
3. Anti-diabetic activities of fucosterol from Pelvetia siliquosa. [J] . Lee YS, Shin KH, Kim BK, Archives of pharmacal research . 2004,第11期

机译：西伯利亚梧桐中岩藻甾醇的抗糖尿病活性。
4. Preparative isolation of Isorhamnetin from stigma maydis using a high-speed countercurrent chromatography [C] . Yun Wei, Xiaoying Cao International Conference on Separation Science and Technology(ICSST'2007); 20071014-16; Beijing(CN) . 2007

机译：使用高速逆流色谱法从柱头可能分离制备异鼠李素
5. New bioactive annonaceous acetogenins from the bark of Annona squamosa, and the use of countercurrent chromatography for their isolation. [D] . Hopp, David Craig. 1997

机译：鳞状Annona的树皮中新的具有生物活性的非制乙酸原素，以及使用逆流色谱法进行分离。
6. Preparative Isolation and Purification of Flavone C-Glycosides from the Leaves of Ficus microcarpa L. f by Medium-Pressure Liquid Chromatography High-Speed Countercurrent Chromatography and Preparative Liquid Chromatography [O] . Xiaohong Wang, Yong Liang, Licai Zhu, -1

机译：中压液相色谱法高速逆流色谱法高速逆流色谱法高速逆流色谱法高速逆流色谱法高速逆流色谱法高速逆流色谱及制备液相色谱法制备纯化及纯化
7. PREPARATIVE ISOLATION AND PURIFICATION OF FLAVONE C-GLYCOSIDES FROM THE LEAVES OF FICUS MICROCARPA L.f BY MEDIUM-PRESSURE LIQUID CHROMATOGRAPHY, HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY, AND PREPARATIVE LIQUID CHROMATOGRAPHY [O] . Xiaohong Wang, Yong Liang, Licai Zhu, 2010

机译：中压液相色谱法，高速逆流色谱法，高速逆流色谱法从Ficus Microcarpa L.F的叶片制备分离和纯化。
8. Centrifugal Countercurrent Partition Chromatography [R] . Fugaki, Y. 1983

机译：离心逆流分配色谱

Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography

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