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首页> 外文期刊>Biochemical Systematics and Ecology >HPLC analysis of the seasonal and diurnal variation of iridoids in cultivars of Antirrhinum majus
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HPLC analysis of the seasonal and diurnal variation of iridoids in cultivars of Antirrhinum majus

机译：山茱Anti品种中鸢尾碱的季节性和昼夜变化的HPLC分析

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摘要

In this paper we show the seasonal and diurnal variation in the content of the four iridoids found ib cultivars of Antirrhinum majus, antirrhinoside, antirrhide, 5-glucosyl-antirrhinoside and linarioside. The seasonal variation in total iridoid content showed a marked bimodal distribution with high total values (around 100mg/g dry matter) early and late in the season and a very low content of all iridoids coinciding with the onset of flowering at the beginning of August. The relative contribution of antirrhinoside was significantly higher before flowering than after bud break. The relative decrease in antirrhinoside counteracted by an increase of antirrhide, which was snigificantly higher after the onset of flowering than before. This pattern indicates a change in biosynthesis, although no explanation can be given to the phenomenon. The diurnal variation showed a variation between 20 and 60mg/g dry weight, but there was no relation to light/darkness conditions, temperature patterns or water content. The analyses were performed by HPLC. The applied method has not previously been used in the quantification of iridoids, but was developed spceifically for the analyses of cultivars of Antirrhinum majus. We have fully validated the method during its development. The limit of detection was calculated to 0.004 mg/ml and the limit of quantification was 0.01 mg/ml.
机译：在本文中，我们显示了在大枣，大黄苷，抗大黄素，5-葡糖基大黄素和利拉那苷中发现的四个鸢尾酮含量的季节性和昼夜变化。总虹彩含量的季节变化显示出明显的双峰分布，在季节的早期和晚期具有较高的总值（约100mg / g干物质），所有虹彩含量非常低，与8月初开始开花相吻合。抗草甘膦苷的相对贡献显着高于花蕾破裂前。抗雌激素苷的相对减少被抗雌激素的增加所抵消，抗雌激素的增加在开花开始后比以前显着更高。这种模式表明生物合成发生了变化，尽管无法对此现象做出任何解释。日变化显示干重在20至60mg / g之间变化，但与明/暗条件，温度模式或水含量无关。通过HPLC进行分析。应用的方法以前没有用于类环烷烃的定量，但是专门用于分析金鱼草的品种。在开发过程中，我们已经对该方法进行了充分验证。计算的检出限为0.004 mg / ml，定量限为0.01 mg / ml。

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