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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >The force dependence of isometric and concentric potentiation in mouse muscle with and without skeletal myosin light chain kinase
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The force dependence of isometric and concentric potentiation in mouse muscle with and without skeletal myosin light chain kinase

机译：有和没有骨骼肌肌球蛋白轻链激酶的小鼠肌肉中等距和同心增强的力依赖性

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The isometric potentiation associated with myosin phosphorylation is force dependent. The purpose of this study was to assess the influence of a pre-existing period of isometric force on the concentric force potentiation displayed by mouse muscles with and without the ability to phosphorylate myosin. We tested isometric (ISO) and concentric (CON) potentiation, as well as concentric potentiation after isometric force (ISO-CON), in muscles from wild-type (WT) and skeletal myosin light chain kinase-deficient (skMLCK-/-) mice. A conditioning stimulus increased (i.e., potentiated) mean concentric force in the ISO-CON and CON conditions to 1.31 +/- 0.02 and 1.35 +/- 0.02 (WT) and to 1.19 +/- 0.02 and 1.21 +/- 0.01 (skMLCK-/-) of prestimulus levels, respectively (data n = 6-8, p < 0.05). No potentiation of mean isometric force was observed in either genotype. The potentiation of mean concentric force was inversely related to relative tetanic force level (P/P-o) in both genotypes. Moreover, concentric potentiation varied greatly within each contraction type and was negatively correlated with unpotentiated force in both genotypes. Thus, although no effect of pre-existing force was observed, strong and inverse relationships between concentric force potentiation and unpotentiated concentric force may suggest an influence of attached and force-generating crossbridges on potentiation magnitude in both WT and skMLCK-/- muscles.
机译：与肌球蛋白磷酸化相关的等距增强是力依赖性的。这项研究的目的是评估预先存在的等轴测力周期对具有和不具有磷酸化肌球蛋白能力的小鼠肌肉所表现出的同心力增强的影响。我们在野生型（WT）和骨骼肌肌球蛋白轻链激酶缺陷（skMLCK-/-）的肌肉中测试了等距（ISO）和同心（CON）增强以及等距受力后的同心增强（ISO-CON）老鼠。在ISO-CON和CON条件下，调节刺激使平均同心力增加（即增强）到1.31 +/- 0.02和1.35 +/- 0.02（WT）以及1.19 +/- 0.02和1.21 +/- 0.01（skMLCK -/-）分别为刺激前水平（资料n = 6-8，p <0.05）。两种基因型均未观察到平均等轴测力的增强。在两种基因型中，平均同心力的增强与相对破伤力水平（P / P-o）成反比。此外，同心增强在每种收缩类型中差异很大，并且在两种基因型中均与未增强力负相关。因此，尽管未观察到预先存在的力的影响，但同心力增强和未增强的同心力之间的强而逆的关系可能表明附着的和产生力的跨桥对WT和skMLCK-/-肌肉中增强强度的影响。

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