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首页> 外文期刊>Functional Plant Biology >Accumulation of soluble carbohydrates, trehalase and sucrose synthase in effective (Fix(+)) and ineffective (Fix(-)) nodules of soybean cultivars that differentially nodulate with Bradyrhizobium japonicum
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Accumulation of soluble carbohydrates, trehalase and sucrose synthase in effective (Fix(+)) and ineffective (Fix(-)) nodules of soybean cultivars that differentially nodulate with Bradyrhizobium japonicum

机译：大豆根瘤菌根瘤菌不同结节的有效（Fix（+））和无效（Fix（-））结节中可溶性碳水化合物，海藻糖和蔗糖合酶的积累

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Roots of soybeans have the ability to form symbioses with nitrogen-fixing rhizobial bacteria to form nitrogen-fixing (Fix(+)) nodules, thus allowing the plant to grow in the absence of mineral nitrogen. Several soybean cultivars from China nodulated normally with Bradyrhizobium japonicum USDA110 spc4, but developed only a few nodules with 61-A-101, another B. japonicum strain. When soybeans were infected with Rhizobium sp. NGR234, ineffective (Fix(-)) nodules that do not fix nitrogen were formed. Plants infected with NGROmeganodD2, a mutant strain overproducing lipo-chitooligosaccharidic nodulation signals ( Nod factors), showed significantly higher numbers of ineffective nodules. Nodules from the different plant - microsymbiont combinations were characterized with respect to their accumulation of soluble carbohydrates and their induction of trehalase and sucrose synthase. These two plant enzymes are known to be nodule-stimulated proteins. Pool sizes of soluble carbohydrates in nodules showed strain-specific alterations in sucrose and trehalose, whereas myo-inositol and pinitol were affected in a more cultivar-specific way. Immunoblots with nodulin-specific antiserum indicated that sucrose synthase is induced in Fix(+) nodules, but undetectable in Fix(-) nodules, indicating a strain-specific induction profile. Trehalase activity in nodules showed a similar strain-specific induction profile. High enzyme activity was measured for nodules harboring the Bradyrhizobium strains, whereas ineffective nodules containing NGR234 exhibited activities in the range of uninfected roots. Nodules induced by NGROmeganodD2 showed increased trehalase activity. A similar induction of trehalase was observed when uninfected roots were treated with Nod factors purified from NGR234. The data obtained are discussed in the context of carbohydrate allocation in nodules and the question of how rhizobial bacteria influence the carbohydrate metabolism of their host plant is addressed.
机译：大豆的根具有与固氮根瘤菌形成共生菌的能力，从而形成固氮（Fix（+））根瘤，从而使植物在没有矿物氮的情况下生长。几个来自中国的大豆品种通常用日本根瘤菌根瘤菌USDA110 spc4进行根瘤形成，但仅用另一个日本根瘤菌菌株61-A-101形成了几个根瘤。当大豆被根瘤菌感染时。 NGR234，不固定氮的无效（Fix（-））结核形成。 NGROmeganodD2（一种过量产生脂-壳寡糖的结节信号（Nod因子）的突变株）感染的植物显示出大量无效结节。从不同植物-微共生蛋白组合的结节就其可溶性碳水化合物的积累及其对海藻糖酶和蔗糖合酶的诱导进行了表征。已知这两种植物酶是结节刺激蛋白。结节中可溶性碳水化合物的库大小显示出蔗糖和海藻糖的菌株特异性改变，而肌醇和松糖醇则以更具体的栽培品种方式受到影响。带有结节蛋白特异性抗血清的免疫印迹表明，蔗糖合酶在Fix（+）结节中被诱导，但在Fix（-）结节中未检测到，表明菌株特异性诱导谱。结节中的海藻糖酶活性显示出相似的菌株特异性诱导曲线。对于带有缓生根瘤菌菌株的根瘤，测定了较高的酶活性，而含有NGR234的无效根瘤在未感染根的范围内表现出活性。 NGROmeganodD2诱导的结节显示海藻糖酶活性增加。当未感染的根用从NGR234纯化的Nod因子处理时，观察到了类似的海藻糖酶诱导作用。在结节中碳水化合物分配的背景下讨论了获得的数据，并讨论了根瘤菌如何影响其宿主植物的碳水化合物代谢的问题。

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