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首页> 外文期刊>Macromolecular Research >Effect of dual growth factor delivery using poly(lactic-co-glycolic acid) mesh on neovascularization in a mouse skin flap model
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Effect of dual growth factor delivery using poly(lactic-co-glycolic acid) mesh on neovascularization in a mouse skin flap model

机译：在小鼠皮肤皮瓣模型中使用聚乳酸-乙醇酸网孔递送双重生长因子对新生血管形成的影响

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Skin flaps have the crucial issue that skin necrosis can occur due to tissue ischemia in the distal parts of flaps. To prevent tissue necrosis, skin flaps need to be supplied with sufficient blood perfusion through quick vascularization in the ischemic area. In this study, we fabricated poly(lactic-co-glycolic acid) (PLGA) meshes that had shaped random or alignments using electrospinning and modified the surface of the PLGA mesh, using 3,4-dihydroxy- L-phenylalanine (L-DOPA), for drug delivery. We carried out X-ray photoelectron spectroscopy and contact angle analysis to confirm the L-DOPA coating on the surface of PLGA meshes. Then, we loaded vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2) on the PLGA meshes for quicker angiogenesis through synergistic effect of the two growth factors. We investigated whether dual growth factor-loaded PLGA meshes on skin flaps promoted angiogenesis during 7-day treatment. When implanted into ICR mice models for skin flaps, the PLGA random mesh led to a result value of significantly smaller than the PLGA aligned mesh in skin flap necrosis and the epidermal thickness with the PLGA random mesh was much thicker than other groups in the necrotic area. Furthermore, coadministration of the two growth factors with the PLGA random meshes resulted in significantly higher blood vessel density than PLGA aligned mesh by ingrowth of host vessels at the edge of the skin flap. These results indicated that dual growth factor-loaded PLGA meshes could contribute to reducing the necrosis by dual growth factor delivery and geometric cues.
机译：皮瓣的关键问题是，由于皮瓣远端的组织缺血会导致皮肤坏死。为了防止组织坏死，需要通过在缺血区域快速血管化为皮瓣提供足够的血液灌注。在这项研究中，我们制作了聚乳酸-乙醇酸共聚物（PLGA）筛网，该筛网使用静电纺丝成形为随机或对齐形状，并使用3,4-二羟基-L-苯丙氨酸（L-DOPA）修饰了PLGA筛网的表面），用于药物输送。我们进行了X射线光电子能谱和接触角分析，以确认PLGA网格表面上的L-DOPA涂层。然后，我们在PLGA网格上加载了血管内皮生长因子（VEGF）和成纤维细胞生长因子2（FGF2），以通过两种生长因子的协同作用更快地进行血管生成。我们调查了在皮瓣上加载双生长因子的PLGA网格在7天的治疗过程中是否促进了血管生成。当将其植入皮瓣的ICR小鼠模型中时，PLGA随机网格的结果值明显小于皮瓣坏死的PLGA对齐网格，并且PLGA随机网格的表皮厚度比坏死区域的其他组要厚得多。此外，两种生长因子与PLGA随机网格的共同施用会导致宿主血管在皮瓣边缘向内生长，从而比PLGA对齐网格明显提高血管密度。这些结果表明，加载双生长因子的PLGA网格可以通过双生长因子传递和几何提示来减少坏死。

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