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首页> 外文期刊>Biochemistry >Probing the interplay between the two steps of group I intron splicing: Competition of exogenous guanosine with omega G
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Probing the interplay between the two steps of group I intron splicing: Competition of exogenous guanosine with omega G

机译：探索第一组内含子剪接两个步骤之间的相互作用：外源鸟苷与欧米茄G的竞争

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摘要

One largely unexplored question about group I intron splicing is how the cleavage and ligation steps of the reaction are coordinated. We describe a simple in vitro trans-splicing model system in which both steps take place, including the exchange of ligands in the guanosine-binding site that must occur between the two steps. Using this model system, we show that the switch is accomplished by modulating the relative affinity of the binding site fur the two ligands, While the terminal guanosine of the intron (omega G) and exogenous guanosine compete for binding during the first step of splicing, no competition is apparent during the second step, when omega G is bound tightly. These results help explain how the ribozyme orchestrates progression through the splicing reaction. In addition to providing a new tool to ask basic questions about RNA catalysis, the trans-splicing model system will also facilitate the development of therapeutically useful group I ribozymes that can repair mutant mRNAs. [References: 50]
机译：关于I组内含子剪接的一个很大程度上未探讨的问题是反应的切割和连接步骤如何协调。我们描述了一个简单的体外转拼模型系统，其中两个步骤都发生，包括必须在两个步骤之间发生的鸟苷结合位点中的配体交换。使用这个模型系统，我们证明了转换是通过调节两个配体的结合位点的相对亲和力来完成的，而内含子的末端鸟苷（ωG）和外源性鸟苷在剪接的第一步中竞争结合，当欧米茄G紧密结合时，在第二步中没有明显的竞争。这些结果有助于解释核酶如何通过剪接反应协调进展。除了提供新的工具来询问有关RNA催化的基本问题外，转拼模型系统还将促进可修复突变体mRNA的具有治疗意义的I类核酶的开发。 [参考：50]

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