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首页> 外文期刊>Mammalia: Morphologie, Biologie Systematique des Mammiferes. >Molecular phylogeny of brush-tailed mice of the genus Calomyscus (Rodentia: Calomyscidae) inferred from mitochondrial DNA sequences (Cox1 gene)
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Molecular phylogeny of brush-tailed mice of the genus Calomyscus (Rodentia: Calomyscidae) inferred from mitochondrial DNA sequences (Cox1 gene)

机译:从线粒体DNA序列(Cox1基因)推论的Calyomyscus属(Rodentia:Caomyscidae)刷尾小鼠的分子系统发育。

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This study examined the genetic divergence of cytochrome oxidase I (Cox1) and the molecular phylogenetic relationships among allopatric populations of five morphospecies of brush-tailed mice (Calomyscus). The genus Calomyscus had long been considered a single species, Calomyscus bailwardi; however, currently, eight geographical species are recognized on the basis of karyotypic, morphologic, and restriction fragment length polymorphism differentiation. Molecular approaches (DNA sequencing) have recently been used to examine the level of genetic differentiation, but this research has principally been restricted to cytochrome b sequences throughout the ranges of C. baluchi and C. hotsoni. To further examine the relationships among species of Calomyscus, we evaluated five morphospecies using DNA sequences. Specimens of Calomyscus were collected from 13 sites in Iran and Pakistan. A total of 637 base pairs of a mitochondrial gene (Cox1) was amplified and sequenced. Analyses using maximum parsimony, maximum likelihood, and Bayesian inference provided similar results and placed the representatives of the five morphospecies into five well-supported clades, each corresponding to one of the recognized morphospecies. Furthermore, our molecular data expanded the known distribution of C. elburzensis from northeast to central Iran (Razavi Khorasan, Northern Khorasan, and Yazd provinces) and identified the Yazd population as a geographic isolate with ties to the Elburz Mountains.
机译:这项研究审查了细胞色素氧化酶I(Cox1)的遗传差异和刷尾小鼠(Calomyscus)的五个形态物种的异源种群之间的分子系统发育关系。 Calomyscus属长期以来一直被认为是单一物种,Balwardicus Calomyscus。然而,目前,根据核型,形态学和限制性片段长度多态性的区分,识别出八个地理物种。最近已经使用分子方法(DNA测序)来检查遗传分化的水平,但是这项研究主要限于在整个棒状杆菌和嗜热梭菌的细胞色素b序列。为了进一步检查加州夜蛾物种之间的关系,我们使用DNA序列评估了5个形态物种。从伊朗和巴基斯坦的13个地点收集了卡柳菌的标本。总共637个碱基对的线粒体基因(Cox1)进行了扩增和测序。使用最大简约性,最大似然和贝叶斯推断进行的分析提供了相似的结果,并将五个形态学的代表放入了五个支撑良好的进化枝中,每个进化枝对应于一种公认的形态学。此外,我们的分子数据将已知的艾伯塔梭菌分布从东北到伊朗中部(拉扎维·霍拉桑,北呼拉桑和亚兹德省),并将亚兹德人口确定为与埃尔伯斯山有联系的地理隔离。

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