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首页> 外文期刊>Biochemistry >DIFFERENT EFFECTS OF HISTONE H1 ON DE NOVO DNA METHYLATION IN VITRO DEPEND ON BOTH THE DNA BASE COMPOSITION AND THE DNA METHYLTRANSFERASE
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DIFFERENT EFFECTS OF HISTONE H1 ON DE NOVO DNA METHYLATION IN VITRO DEPEND ON BOTH THE DNA BASE COMPOSITION AND THE DNA METHYLTRANSFERASE

机译：组蛋白H1对DNA从头组成和DNA甲基转移酶体外DE NOVO DNA甲基化的不同影响

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摘要

We have characterized the inhibition exerted by histone H1 on the activity of human placenta DNA (cytosine-5-)-methyltransferase. Our experiments demonstrate that the extent of inhibition depends on the DNA base composition, AT-rich substrates being more severely affected than GC-rich substrates and CpG-rich islands. With bacterial SssI methylase, the effect is completely reversed since its activity on AT-rich substrates undergoes a 4-5-fold stimulation upon the addition of H1. Poly(L-lysine) mimicks H1 effects, suggesting an essential role of lysine residues in both the inhibitory and stimulatory effects of H1. By comparison of the different behaviors of the two enzymes, the inhibitory effect over the eukaryotic enzyme might be accounted for by hypothesizing a competition between minor groove-binding motifs (SPKK-like) present in placenta methylase as well as in histone H1.
机译：我们已经表征了组蛋白H1对人胎盘DNA（cytosine-5-）-甲基转移酶活性的抑制作用。我们的实验表明抑制程度取决于DNA碱基组成，富含AT的底物比富含GC的底物和富含CpG的岛受到的影响更大。使用细菌SssI甲基化酶，由于添加了H1后，其对富含AT的底物的活性受到了4-5倍的刺激，因此这种作用被完全逆转。聚（L-赖氨酸）模仿H1的影响，表明赖氨酸残基在H1的抑制和刺激作用中的重要作用。通过比较这两种酶的不同行为，可以通过假设存在于胎盘甲基化酶和组蛋白H1中的小沟结合基序（SPKK样）之间的竞争来解释对真核酶的抑制作用。

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