...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Marine genomics >Molecular cloning of calnexin and calreticulin in the Pacific oyster Crassostrea gigas and its expression in response to air exposure
【24h】

Molecular cloning of calnexin and calreticulin in the Pacific oyster Crassostrea gigas and its expression in response to air exposure

机译：太平洋牡蛎Crassostrea gigas中钙粘蛋白和钙网蛋白的分子克隆及其在空气中的表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Calnexin (CNX) and calreticulin (CRT) are endoplasmic reticulum (ER) chaperones. CNX is a type I transmembrane protein and CRT is a soluble CNX homologue. In the ER, CNX and CRT are important for Ca~2+ homeostasis and protein maturation. Here, we describe the full-length cDNA of the first mollusk CNX (cgCNX) and a second mollusk CRT (cgCRT) from the oyster Crassostrea gigas. CgCNX, containing 3255 bp, was composed of a 1764 bp open reading frame (ORF) that encodes a 588-amino acid protein. CgCRT, containing 1727 bp, was composed of a 1242 bp ORF that encodes a 414-amino acid protein. CgCNX and cgCRT contains an N-terminal 21- and 16-amino acid sequence, respectively, which is characteristic of a signal sequence. At the C-terminus, cgCRT also contains the KDEL (-Lys-Asp-Glu-Leu) peptide motif suggesting that cgCRT localizes in the ER Northern blot analysis showed that both cgCNX and cgCRT mRNAs are induced by air exposure. The expression patterns of cgCNX mRNA differed from those of cgCRT during air exposure. This suggests that these two molecular chaperones have different roles in the response to air exposure.
机译：钙粘蛋白（CNX）和钙网蛋白（CRT）是内质网（ER）伴侣。 CNX是I型跨膜蛋白，CRT是可溶性CNX同源物。在急诊室，CNX和CRT对于Ca〜2 +稳态和蛋白质成熟很重要。在这里，我们描述了牡蛎Crassostrea gigas的第一个软体动物CNX（cgCNX）和第二个软体动物CRT（cgCRT）的全长cDNA。 CgCNX包含3255 bp，由一个1764 bp的开放阅读框（ORF）组成，该开放阅读框编码一个588个氨基酸的蛋白质。包含1727 bp的CgCRT由一个1242 bp的ORF组成，编码一个414个氨基酸的蛋白质。 CgCNX和cgCRT分别包含一个N末端的21和16个氨基酸序列，该序列是信号序列的特征。在C末端，cgCRT还包含KDEL（-Lys-Asp-Glu-Leu）肽基序，这表明cgCRT位于ER Northern印迹分析中，表明cgCNX和cgCRT mRNA均受空气暴露诱导。暴露于空气中时，cgCNX mRNA的表达模式与cgCRT的表达模式不同。这表明这两种分子伴侣在对空气暴露的反应中具有不同的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号