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首页> 外文期刊>Mass Spectrometry Reviews >PROTEOMICS OF REGULATED SECRETORY ORGANELLES
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PROTEOMICS OF REGULATED SECRETORY ORGANELLES

机译：监管证券组织的蛋白质组学

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摘要

Regulated secretory organelles are important subcellular structures of living cells that allow the release in the extracellular space of crucial compounds, such as hormones and neurotransmitters. Therefore, the regulation of biogenesis, trafficking, and exocytosis of regulated secretory organelles has been intensively studied during the last 30 years. However, due to the large number of different regulated secretory organelles, only a few of them have been specifically characterized. New insights into regulated secretory organelles open crucial perspectives for a better comprehension of the mechanisms that govern cell secretion. The combination of subcellular fractionation, protein separation, and mass spectrometry is also possible to study regulated secretory organelles at the proteome level. In this review, we present different strategies used to isolate regulated secretory organelles, separate their protein content, and identify the proteins by mass spectrometry. The biological significance of regulated secretory organelles-proteomic analysis is discussed as well.
机译：调节的分泌细胞器是活细胞的重要亚细胞结构，可以使重要化合物（如激素和神经递质）在细胞外释放。因此，在过去的30年中，已经深入研究了调节性分泌细胞器的生物发生，运输和胞吐作用的调节。但是，由于存在大量不同的受调节的分泌细胞器，因此仅对其中的几个进行了特殊表征。对调节型分泌细胞器的新见解为更好地理解控制细胞分泌的机制打开了重要的视野。亚细胞分级分离，蛋白质分离和质谱的结合也可以在蛋白质组学水平上研究受调节的分泌细胞器。在这篇综述中，我们提出了用于分离受调控的分泌细胞器，分离其蛋白质含量并通过质谱鉴定蛋白质的不同策略。还讨论了调节性分泌细胞器蛋白质组学分析的生物学意义。

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