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Reorganization of low-molecular-weight fraction of plasma proteins in the annual cycle of cyprinidae

机译：鲤科动物一年周期中血浆蛋白低分子量组分的重组

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Reorganization of the low-molecular-weight fraction of cyprinid plasma was analyzed using various electrophoretic techniques (disc electrophoresis, electrophoresis in polyacrylamide concentration gradient, in polyacrylamide with urea, and in SDS-polyacrylamide). The study revealed coordinated changes in the low-molecular-weight protein fractions with seasonal dynamics and related reproductive rhythms of fishes. We used cultured species of the Cyprinidae family with sequenced genomes for the detection of these interrelations in fresh-water and anadromous cyprinid species. The common features of organization of fish low-molecular-weight plasma protein fractions made it possible to make reliable identification of their proteins. MALDI mass-spectrometry analysis revealed the presence of the same proteins (hemopexin, apolipoproteins, and serpins) in the low-molecular-weight plasma fraction in wild species and cultured species with sequenced genomes (carp, zebrafish). It is found that the proteins of the first two classes are organized as complexes made of protein oligomers. Stoichiometry of these complexes changes in concordance with the seasonal and reproductive rhythms.
机译：使用多种电泳技术（圆盘电泳，聚丙烯酰胺浓度梯度电泳，聚丙烯酰胺和尿素以及SDS-聚丙烯酰胺电泳）分析了塞浦路斯血浆的低分子量部分的重组。该研究揭示了低分子量蛋白质组分随季节动态和相关的生殖节律的协调变化。我们使用了具有测序基因组的Cyprinidae家族的培养物种来检测淡水和无水鲤科鱼类中的这些相互关系。鱼的低分子量血浆蛋白组分的共同特征使其可以可靠地鉴定其蛋白。 MALDI质谱分析揭示了野生物种和具有测序基因组（鲤鱼，斑马鱼）的培养物种的低分子量血浆组分中存在相同的蛋白质（hemopexin，载脂蛋白和丝氨酸蛋白酶抑制剂）。发现前两类蛋白质被组织成由蛋白质寡聚物制成的复合物。这些配合物的化学计量根据季节和生殖节律而变化。

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