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首页> 外文期刊>Materials transactions >Non-Contact Surface Morphology Analysis of CO_2 Laser-Irradiated Teeth by Scanning Electron Microscope and Confocal Laser Scanning Microscope
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Non-Contact Surface Morphology Analysis of CO_2 Laser-Irradiated Teeth by Scanning Electron Microscope and Confocal Laser Scanning Microscope

机译：扫描电子显微镜和共聚焦激光扫描显微镜对CO_2激光辐照牙齿的非接触表面形态分析

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摘要

A newly developed scanning electron microscope (SEM) installed with a 3D analyzer and a confocal laser scanning microscope (CLSM) were used to evaluate the images and surface profiles of enamel and dentin after CO_2 laser irradiation. The surface roughness (Ra) was measured and the results were correlated with a stylus profilometer. Raman analysis was done and the laser irradiation effect after acid etching was also examined. Human intact extracted teeth were vertically sectioned. The laser was applied perpendicularly to non-etched and 35 percent phosphoric acid-etched teeth. The enamel resulted in a crater-like surface. The material was removed in the places where hydroxyapatite crystallites ran parallel to the irradiated surface. Non-etched dentin showed little change after irradiation, with some cracks mostly in the peritubular dentin; whereas in acid-etched dentin there was evaporation of collagen and melting of hydroxyapatite. The SEM and CLSM showed similar profiles and different image contrast. Ra levels obtained by the SEM and CLSM were similar- to that obtained with the stylus profilometer. Raman analysis showed that bands of organic collagen matrix on dentin were lost and broad peaks due to carbon were observed. Both the SEM and CLSM provided non-contact evaluation of structural changes of teeth by laser irradiation through surface analysis in selected microareas, which was not possible using the stylus profilometer. Dentin showed more structural changes on the acid-etched surface by irradiation than on the non-etched one.
机译：安装有3D分析仪和共聚焦激光扫描显微镜（CLSM）的新开发的扫描电子显微镜（SEM）用于评估CO_2激光照射后牙釉质和牙本质的图像和表面轮廓。测量表面粗糙度（Ra），并将结果与​​测针轮廓仪相关联。进行了拉曼分析，还检查了酸蚀刻后的激光照射效果。将人类完整的拔出牙齿垂直切开。将激光垂直应用于未蚀刻和35％磷酸蚀刻的牙齿。珐琅质形成火山口状的表面。在羟基磷灰石微晶平行于被辐照表面的地方除去了该材料。未腐蚀的牙本质在照射后几乎没有变化，一些裂纹主要发生在肾小管周围的牙本质中。而在酸蚀的牙本质中，胶原蛋白蒸发并且羟磷灰石熔化。 SEM和CLSM显示出相似的轮廓和不同的图像对比度。通过SEM和CLSM获得的Ra水平类似于使用测针轮廓仪获得的Ra水平。拉曼分析显示，牙本质上的有机胶原基质带丢失，并且观察到由于碳引起的宽峰。 SEM和CLSM都可以通过在选定的微区域中进行表面分析，通过激光辐照来对牙齿的结构变化进行非接触式评估，这是使用测针轮廓仪无法实现的。牙本质在经酸腐蚀的表面上显示出比未经腐蚀的表面更多的结构变化。

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