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Methods of genome engineering: a new era of molecular biology

机译:基因组工程方法:分子生物学的新纪元

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Genome sequencing now progressing much faster than our understanding of the majority of gene functions. Studies of physiological functions of various genes would not be possible without the ability to manipulate the genome. Methods of genome engineering can now be used to inactivate a gene to study consequences, introduce heterologous genes into the genome for scientific and biotechnology applications, create genes coding for fusion proteins to study gene expression, protein localization, and molecular interactions, and to develop animal models of human diseases to find appropriate treatment. Finally, genome engineering might present the possibility to cure hereditary diseases. In this review, we discuss and compare the most important methods for gene inactivation and editing, as well as methods for incorporation of heterologous genes into the genome.
机译:现在,基因组测序的进展比我们对大多数基因功能的了解要快得多。没有操纵基因组的能力,就不可能研究各种基因的生理功能。现在可以使用基因组工程方法来灭活基因以研究后果,将异源基因引入基因组以用于科学和生物技术应用,创建编码融合蛋白的基因以研究基因表达,蛋白质定位和分子相互作用,并开发动物寻找合适的人类疾病模型。最后,基因组工程可能提供治愈遗传性疾病的可能性。在这篇综述中,我们讨论和比较了最重要的基因失活和编辑方法,以及将异源基因整合到基因组中的方法。

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