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首页> 外文期刊>Biochemistry >A MODEL OF THE IRON RESPONSIVE ELEMENT RNA HAIRPIN LOOP STRUCTURE DETERMINED FROM NMR AND THERMODYNAMIC DATA
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A MODEL OF THE IRON RESPONSIVE ELEMENT RNA HAIRPIN LOOP STRUCTURE DETERMINED FROM NMR AND THERMODYNAMIC DATA

机译：核磁共振和热力学数据确定铁反应性元素RNA发夹环结构的模型

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摘要

The iron responsive element (IRE) is a conserved RNA structure that is found in the 5' UTR of ferritin mRNA and in the 3' UTR of transferrin receptor mRNA. It is the binding site of the iron responsive protein (IRP), and the interaction is part of the regulation of cellular iron metabolism The IRE six-nucleotide hairpin loop, 5'C(1)A(2)G(3)U(4)G(5)N(6), is conserved in sequence, and mutations have shown that it is required for IRP binding. On the basis of the thermodynamic and NMR experiments utilized here, the IRE loop structure 5'C(1)A(2)G(3)U(4)G(5)C(6), is described in detail. Measurements of loop stability show that it has 2.9 kcal/mol more free energy than predicted. Nh4R data suggest that there is hydrogen bonding between C1 and G5 in a tertiary interaction across the loop. A model structure, produced by MC-SYM/energy minimization, illustrates the conformational flexibility of U4 and C6, which appear to exhibit considerable local motion in solution. NMR data indicate that the position of G3 is not well defined, leading to two families of loop structures.
机译：铁反应元件（IRE）是一种保守的RNA结构，存在于铁蛋白mRNA的5'UTR和运铁蛋白受体mRNA的3'UTR中。它是铁反应蛋白（IRP）的结合位点，并且相互作用是细胞铁代谢调节的一部分。IRE六核苷酸发夹环5'C（1）A（2）G（3）U（ 4）G（5）N（6）在序列上是保守的，突变表明它是IRP结合所必需的。根据此处使用的热力学和NMR实验，将详细描述IRE回路结构5'C（1）A（2）G（3）U（4）G（5）C（6）。回路稳定性的测量结果表明，它的自由能比预期高2.9 kcal / mol。 Nh4R数据表明，在整个环的三次相互作用中，C1和G5之间存在氢键。由MC-SYM /能量最小化产生的模型结构说明了U4和C6的构象灵活性，它们在溶液中表现出可观的局部运动。 NMR数据表明，G3的位置没有明确定义，导致形成两个环状结构家族。

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