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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >Collagen type I, III and V differently modulate synthesis and activation of matrix metalloproteinases by cultured rabbit periosteal fibroblasts.
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Collagen type I, III and V differently modulate synthesis and activation of matrix metalloproteinases by cultured rabbit periosteal fibroblasts.

机译：I，III和V型胶原蛋白通过培养的兔骨膜成纤维细胞不同地调节基质金属蛋白酶的合成和激活。

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摘要

In the present study we investigated whether the collagen types I, III and V affect the activity of fibroblasts obtained from rabbit periosteum. The cells were cultured on plates either or not coated with different amounts of collagen type I, III or V and analyzed for their attachment, DNA synthesis and the expression and activity of matrix metalloproteinases (MMPs). Our data show that the three collagen types promoted attachment and spreading of the cells and stimulated DNA synthesis when used in relatively low concentrations. High concentrations of type V-but not of type I or III-proved to inhibit thymidine incorporation. The expression and activity of matrix metalloproteinase 1 (MMP-1; interstitial collagenase) decreased under the influence of relatively low amounts of collagen (<40 microg/well), whereas higher levels increased its release. Matrix metalloproteinase 2 (MMP-2; gelatinase A) was up-regulated by the different types of collagen; the active fraction of stromelysin-1 (MMP-3) decreased. Accordingly, the mRNA expression of MMP-1 and -3 were reduced. The expression of MMP-2 mRNA, however, proved to be unaffected. Blocking antibodies to beta(1)-integrin or echistatin increased the level of MMP-1 but had no effect on MMP-2. All parameters tested were similarly affected by type I and III collagen, whereas the effect of type V was always less. We conclude that the collagen types I, III and V provide different sets of signals for fibroblasts that differently modulate their proliferation and MMP expression.
机译：在本研究中，我们调查了I，III和V型胶原蛋白是否影响从兔骨膜获得的成纤维细胞的活性。将细胞培养在平板上，无论平板是否被不同量的I，III或V型胶原包被，并对其附着，DNA合成以及基质金属蛋白酶（MMP）的表达和活性进行分析。我们的数据表明，当以相对较低的浓度使用时，三种胶原蛋白类型可促进细胞的附着和扩散，并刺激DNA合成。高浓度的V型（但不是I型或III型）被证明可以抑制胸苷的掺入。基质金属蛋白酶1（MMP-1;间质胶原酶）的表达和活性在相对少量的胶原蛋白（<40微克/孔）的影响下下降，而较高的水平则增加了其释放。基质金属蛋白酶2（MMP-2;明胶酶A）被不同类型的胶原蛋白上调。 Stromelysin-1（MMP-3）的活性分数降低。因此，MMP-1和-3的mRNA表达降低。然而，MMP-2 mRNA的表达被证明不受影响。阻断针对beta（1）整合素或echistatin的抗体可提高MMP-1的水平，但对MMP-2没有影响。测试的所有参数同样受到I型和III型胶原蛋白的影响，而V型的影响始终较小。我们得出的结论是，I型，III型和V型胶原蛋白为成纤维细胞提供了不同的信号集，可以不同地调节其增殖和MMP表达。

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