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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >Expression and secretion of rat SPAM1(2B1 or PH-20) in the epididymis: role of testicular lumicrine factors.
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Expression and secretion of rat SPAM1(2B1 or PH-20) in the epididymis: role of testicular lumicrine factors.

机译：大鼠SPAM1（2B1或PH-20）在附睾中的表达和分泌：睾丸发光因子的作用。

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摘要

Rat sperm surface antigen Sperm Adhesion Molecule1, SPAM1 (a.k.a. 2B1 or PH-20) is a plasma membrane-bound glycoprotein with hyaluronidase activity and putative roles during fertilization. Previously the antigen was thought to be testis-specific but recently it has been shown to be synthesized in the epididymis (mouse, macaque and human). Using the efferent ductule ligated (EDL) rat as a model to produce a sperm-free androgen-maintained epididymis, we have examined the factors regulating the expression of epididymal 2B1. RT-PCR and in situ transcript hybridization (ISH) studies showed that 2B1 mRNA is transcribed in the principal cells in all three regions of the epididymis. Its cognate protein was also detected by Western blot analysis in sperm-free cytosols from normal epididymis and found to undergo endoproteolytic cleavage into 2 subunits of similar size to the sperm-bound form. Immunohistochemistry with a monoclonal antibody to 2B1 confirmed that the protein is present in the epididymal epitheliumand luminal secretions. The intensity of staining was much stronger in the sperm-free EDL epididymis than that in the normal (sperm-present) epididymis. The protein was shown to have hyaluronidase activity at neutral pH and both its quantity and activity appeared to be greater in the EDL epididymis. It is suggested that a soluble form of SPAM1 glycoprotein is synthesized and released in the epididymis and that in addition to androgens, its regulation may involve a cross-talk between the tubule epithelium and lumicrine factors, the latter possibly of testicular origin.
机译：大鼠精子表面抗原Sperm Adhesion Molecule1，SPAM1（又称2B1或PH-20）是与质膜结合的糖蛋白，具有透明质酸酶活性，在受精过程中具有假定的作用。以前，该抗原被认为是睾丸特异性的，但最近已证明它是在附睾（小鼠，猕猴和人）中合成的。以传出小管结扎（EDL）大鼠为模型，以产生无精子的雄激素维持性附睾，我们研究了调节附睾2B1表达的因素。 RT-PCR和原位转录杂交（ISH）研究表明2B1 mRNA在附睾所有三个区域的主要细胞中均有转录。还通过蛋白质印迹分析在正常附睾的无精子细胞质中检测到其同源蛋白，发现其内切蛋白水解为2个亚单位，大小与精子结合形式相似。用针对2B1的单克隆抗体进行免疫组织化学证实，该蛋白存在于附睾上皮和管腔分泌物中。无精子的EDL附睾的染色强度比正常的（有精子的）附睾的染色强度要强。该蛋白质显示在中性pH下具有透明质酸酶活性，并且在EDL附睾中其数量和活性均更高。有人认为，SPAM1糖蛋白的可溶性形式是合成的，并在附睾中释放，除雄激素外，它的调节可能涉及肾小管上皮和发光因子之间的串扰，后者可能是睾丸来源。

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