...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Multiple states of beta-sheet peptide protegrin in lipid bilayers
【24h】

Multiple states of beta-sheet peptide protegrin in lipid bilayers

机译：脂双层中β-片状肽protegrin的多种状态

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protegrin-1 (PG-1), a beta-sheet antimicrobial peptide, was studied in aligned lipid bilayers by oriented circular dichroism (OCD), All of its spectra measured in a variety of lipid compositions were linear superpositions of two primary basis spectra, indicating that PG-1 existed in two different states in membranes. We designated these as state S and state I. The state assumed by PG-I was strongly influenced by lipid composition, peptide concentration, and hydration condition. We have previously reported that the helical peptides, alamethicin and magainin, also exhibit two distinct OCD basis spectra-one corresponding to surface adsorption with the helix parallel to the bilayer and the other with perpendicular transbilayer insertion. States S and I of PG-1 may correspond to the surface state and the insertion state of alamethicin, since they show a similar dependence on lipid composition, peptide concentration, and hydration condition. Nonoriented CD spectra obtained from vesicle, micelle, and solution preparations are not linear superpositions of the basis spectra of the states S and I. This indicates that a molecular orientation change alone is insufficient to describe the S <-> I transition. Rather, a more complicated process is taking place, perhaps involving a change in the hydrogen bonding pattern of the backbone. Although the structural basis of the OCD spectra remains to be determined, the discovery of two distinct states can provide information about dynamic changes of PG-1 in membranelike environments, properties undoubtedly related to its antimicrobial and cytotoxic effects. [References: 40]
机译：Protegrin-1（PG-1）是一种β-折叠抗菌肽，通过定向圆二色性（OCD）在对齐的脂质双层中进行了研究，其在各种脂质成分中测得的所有光谱均为两个主要基础光谱的线性叠加，表明PG-1以两种不同状态存在于膜中。我们将它们指定为状态S和状态I。PG​​-1假定的状态受脂质组成，肽浓度和水合条件的强烈影响。我们以前曾报道过，螺旋肽，乐果霉素和麦胶苷也表现出两个不同的OCD基础光谱，一个对应于表面吸附，螺旋平行于双层，另一个吸附于垂直跨双层插入。 PG-1的状态S和I可能对应于乐果霉素的表面状态和插入状态，因为它们对脂质成分，肽浓度和水合条件的依赖性相似。从囊泡，胶束和溶液制备物中获得的非取向CD光谱不是状态S和I的基本光谱的线性叠加。这表明仅分子取向的变化不足以描述S-I跃迁。而是发生了更复杂的过程，可能涉及主链氢键键合模式的改变。尽管OCD光谱的结构基础尚待确定，但发现两种不同状态可以提供有关PG-1在膜状环境中动态变化的信息，其性质无疑与其抗微生物和细胞毒性作用有关。 [参考：40]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号