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首页> 外文期刊>Medical Physics >Reduction of excitation light leakage to improve near-infrared fluorescence imaging for tissue surface and deep tissue imaging.
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Reduction of excitation light leakage to improve near-infrared fluorescence imaging for tissue surface and deep tissue imaging.

机译：减少激发光泄漏，以改善用于组织表面和深层组织成像的近红外荧光成像。

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PURPOSE: Fluorescence-enhanced optical imaging using near-infrared (NIR) light developed for in vivo molecular targeting and reporting of various diseases provides promising opportunities for diagnostic imaging. However, the measurement sensitivity of NIR fluorescence (NIRF) optical imaging systems is limited by the leakage of the strong backscattered excitation light through rejection filters. In this article, the authors present a systematic method for improving sensitivity and validating the NIRF optical imager currently used for clinical imaging of human lymphatic function. METHODS: The proposed systemic method consists of an appropriate filter combination and a collimation optics adapted to an NIRF optical imager. The spectral contributions were first assessed due to the excitation light backscattered from the tissue and from non-normal-incidence of the excitation light on the optical filters used in the authors' NIRF clinical imaging system. Then two tests were conducted to assess the system with and without the components of appropriate filters combination and collimation optics using: (1) a phantom to evaluate excitation light leakage as a function of target depth and (2) deployment in an actual human study. RESULTS: The phantom studies demonstrate as much as two to three orders of magnitude reduction in the transmission ratio, indicating that the excitation light leakage can be reduced upon using the appropriate filter combination and collimation optics while an in vivo investigatory human study confirms improved imaging. CONCLUSIONS: The method for reducing the excitation light leakage is presented for validating collected signals for fluorescence imaging.
机译：目的：利用近红外（NIR）光开发的荧光增强光学成像技术，用于体内分子靶向和各种疾病的报告，为诊断成像提供了广阔的机遇。但是，NIR荧光（NIRF）光学成像系统的测量灵敏度受到强反向散射激发光通过滤镜的泄漏的限制。在本文中，作者提出了一种用于提高灵敏度和验证当前​​用于人体淋巴功能临床成像的NIRF光学成像仪的系统方法。方法：建议的系统方法由适当的滤光片组合和适用于NIRF光学成像仪的准直光学系统组成。首先评估光谱贡献是由于激发光从组织向后散射，以及激发光在作者的NIRF临床成像系统中使用的光学滤光片上的非正常入射。然后进行了两项测试，以评估使用或不使用适当滤镜组合和准直光学系统组件的系统：（1）幻像，以评估激发光泄漏与目标深度的关系;（2）在实际的人体研究中。结果：幻像研究表明透射率降低了多达2到3个数量级，表明使用适当的滤光片组合和准直光学器件可以减少激发光的泄漏，而一项体内研究的人类研究证实可以改善成像。结论：提出了减少激发光泄漏的方法，用于验证荧光成像采集的信号。

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