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首页> 外文期刊>Free radical research >Effects of an oxidizing agent (hydrogen peroxide) on the glutathione system in epidermal cells of Allium cepa L. investigated by histochemical staining.
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Effects of an oxidizing agent (hydrogen peroxide) on the glutathione system in epidermal cells of Allium cepa L. investigated by histochemical staining.

机译：通过组织化学染色研究了氧化剂（过氧化氢）对洋葱皮细胞中谷胱甘肽系统的影响。

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摘要

The glutathione system in epidermal cells of Allium cepa L. was measured by a quantitative image analysis method, using histochemical staining by monochlorobimane. Blockage of thiol groups with N-ethylmaleimide decreased fluorescence to a small rest fluorescence of maximum 5%. Pre-treatment with dithiothreitol increased fluorescence yield in all cells compared to the monochlorobimane treatment alone. These results correspond to the blockage or the reduction of the total pool of glutathione in the cells. After treatment with N-ethylmaleimide and subsequent incubation with dithiothreitol followed by labelling with bimane a low fluorescence yield was observed which correspond to the proportion of GSSG. Specimens that were incubated in H2O2 prior to the treatments described above exhibited a decrease in total glutathione and in reduced glutathione and an increase in the proportion of GSSG compared to the control.
机译：通过定量图像分析方法，使用一氯二苯胺进行的组织化学染色，通过定量图像分析方法测量了葱属的表皮细胞中的谷胱甘肽系统。用N-乙基马来酰亚胺阻断硫醇基团使荧光减少至最大5％的小剩余荧光。与单独的单氯双马胺处理相比，用二硫苏糖醇预处理可以提高所有细胞的荧光产量。这些结果对应于细胞中谷胱甘肽总库的阻滞或减少。在用N-乙基马来酰亚胺处理并随后与二硫苏糖醇一起温育，然后用双烯丙基标记后，观察到较低的荧光产率，其对应于GSSG的比例。与对照相比，在上述处理之前在H2O2中孵育的标本显示总谷胱甘肽减少，谷胱甘肽减少和GSSG比例增加。

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