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Extraction of polyphenols from Trewia nudiflora L. and its antioxidant activity

机译:T藜多酚的提取及其抗氧化活性

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Total phenolic content (TPC), specific polyphenols (Gallic, ellagic and protocatechuic acid), antioxidant activity (AOA), free radical scavenging activity (FRSA), lipid peroxidation (LPO), inhibition of nitroblue tetrazolium chloride (NBT) reduction caused by superoxide anions and reducing power (RP) through various extraction optimization parameters using solvents of different polarity, time, temperature and pH variation were evaluated in fruits, leaves, twigs and bark of Trewia nudiflora. The extraction parameters exhibited a broad range of TPC in plant parts varying from 7.9-668.6 mg/g gallic acid equivalent (GAE), AOA 14.6-82.9% and RP 0.47-7.2 ASE/ ml. FRSA, measured as IC50 in various antioxidant assays ranged from 0.014-4.1 mg/ml. The extracts of all plant parts showed significant protective effect against Fenton's reaction on supercoiled plasmid DNA pUC 18 assayed by agarose gel electrophoresis. Fruits were found to possess the highest TPC, AOA and FRSA in all the tested models, with reduction in the activity, in order of fruits > leaves > twigs > bark.
机译:总酚含量(TPC),特定的多酚(没食子酸,没食子酸和原儿茶酸),抗氧化剂活性(AOA),自由基清除活性(FRSA),脂质过氧化(LPO),抑制过氧化氢导致的硝基蓝四唑氯化物(NBT)还原在T,果实,嫩枝和树皮中,使用不同极性,时间,温度和pH值变化的溶剂,通过各种萃取优化参数对阴离子和还原力(RP)进行了评估。提取参数在植物部位显示出广泛的TPC范围,范围从7.9-668.6 mg / g没食子酸当量(GAE),AOA 14.6-82.9%和RP 0.47-7.2 ASE / ml。 FRSA,在各种抗氧化剂分析中以IC50衡量,范围为0.014-4.1 mg / ml。通过琼脂糖凝胶电泳测定,所有植物部分的提取物对芬顿对超螺旋质粒DNA pUC 18的反应均显示出显着的保护作用。在所有测试模型中,发现水果具有最高的TPC,AOA和FRSA,且活性降低,依次为水果>树叶>嫩枝>树皮。

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