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Micropropagation, qualitative phytochemical analysis and antioxidant potential of Indigofera aspalathoides Vahl. ex. DC.

机译:靛蓝Indigofera aspalathoides Vahl的微繁殖,定性植物化学分析和抗氧化潜力。例如DC。

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Indigofera aspalathoides Vahl. ex. DC., which belongs to the family Fabaceae, is popularly known as “Wiry Indigo”. In the present study, effective tissue culture protocol of I. aspalathoides was performed by callus induction from different explantssuch as node and root. MS medium supplemented with various concentrations and combinations of plant growth regulators was used. Interestingly, maximum callus induction from root (92%) and nodal explants (89%) was observed in MS medium amended with NAA (0.5 mg/L). Maximum shooting and rooting (95%) from nodal explants were observed on IBA (1.0 mg/L) + BAP (1.5 mg/L). MS medium supplemented with BAP (1.0 mg/L) showed maximum (9P/o) of multiple shoots from organogenic calli. Qualitative phytochemical analysis on methanolic extracts of both wild plant and calli indicated the presence of alkaloids, flavonoids, glycosides, carbohydrates, fixed oils, saponins, proteins and amino acids, phytosterols and phenols. The strong DPPH free radical scavenging activityof methanolic extract of callus showed IC_(50)value of 20.26 ng/mL followed by wild plant 67.23 mug/mL when compared with that of standard ascorbic acid (8.24 mug/mL). [Medicinal Plants 2015; 7(2) : 95-102]
机译:Indigofera aspalathoides Vahl。例如DC。,属于Fabaceae科,通常被称为“ Wiry Indigo”。在本研究中,通过不同结节和根等外植体的愈伤组织诱导进行有效的I. aspalathoides组织培养方案。使用添加了不同浓度的MS培养基以及植物生长调节剂的组合。有趣的是,在用NAA(0.5 mg / L)改良的MS培养基中,观察到根部(92%)和结节外植体(89%)的最大愈伤组织诱导。在IBA(1.0 mg / L)+ BAP(1.5 mg / L)上观察到来自节点外植体的最大射击和生根(95%)。补充有BAP(1.0 mg / L)的MS培养基显示来自器官发生性愈伤组织的多次芽的最大量(9P / o)。对野生植物和愈伤组织的甲醇提取物进行的定性植物化学分析表明,存在生物碱,类黄酮,糖苷,碳水化合物,固定油,皂苷,蛋白质和氨基酸,植物甾醇和酚。愈伤组织的甲醇提取物具有很强的DPPH自由基清除活性,与标准抗坏血酸(8.24马克/毫升)相比,IC_(50)值为20.26 ng / mL,其次是野生植物67.23马克/ mL。 [2015年药用植物; 7(2):95-102]

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