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首页> 外文期刊>Medycyna Weterynaryjna >Comparison of growth of Mycobacterium avium subsp paratuberculosis on three microbiological media [Polish]
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Comparison of growth of Mycobacterium avium subsp paratuberculosis on three microbiological media [Polish]

机译：在三种微生物培养基上鸟分枝杆菌副结核亚种的生长比较[波兰]

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摘要

Mycobacterium avium subsp. paratuberculosis (MAP) growth is difficult under laboratory conditions. Despite significant progress in research on the isolation of MAP, the only method to reveal viable cells in samples is cultivation on microbiological media. O.I.E recommends three media for isolation and cultivation of MAP: Herrold egg yolk medium (HEYM), Lowenstein-Jensen medium and Middlebrook medium, all of them with the addition of mycobactin J. The aim of the study was to compare the rapidity and intensity of the growth of MAP isolated from contaminated milk samples on the above-mentioned media with and without the addition of antibiotics. The fastest growth of MAP (after 5 weeks of incubations) was obtained on HEYM slops, a little slower MAP growth was on Lowenstein-Jensen slops (6-9 weeks). There was no growth on Middlebrook medium with antibiotics after 16 weeks of incubation. Chemical preparation and decontamination of milk samples allow for the application of microbiological media without antibiotics, which increases the time of MAP growth. [References: 22]
机译：鸟分枝杆菌亚种在实验室条件下，副结核病（MAP）很难生长。尽管在MAP分离方面的研究取得了重大进展，但揭示样品中活细胞的唯一方法是在微生物培养基上进行培养。 OIE建议使用三种分离和培养MAP的培养基：Herrold蛋黄培养基（HEYM），Lowenstein-Jensen培养基和Middlebrook培养基，并添加霉菌素J。该研究的目的是比较MAP的快速性和强度。在添加和不添加抗生素的情况下，从上述污染的牛奶样品中分离出的MAP的生长情况。在HEYM污泥上，MAP的生长最快（孵育5周后），在Lowenstein-Jensen污泥上（6-9周），MAP的生长稍慢。孵育16周后，含抗生素的Middlebrook培养基无生长。牛奶样品的化学制备和去污允许使用不含抗生素的微生物培养基，这会增加MAP的生长时间。 [参考：22]

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