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首页> 外文期刊>Medycyna Weterynaryjna >Effect of packaging form and methods of boar semen freezing on chosen reproductive indexes of sowsOriginal Title (non-English) Effect of packaging form and methods of boar semen freezing on chosen reporductive indexes of sows [Polish]
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Effect of packaging form and methods of boar semen freezing on chosen reproductive indexes of sowsOriginal Title (non-English) Effect of packaging form and methods of boar semen freezing on chosen reporductive indexes of sows [Polish]

机译：冻精包装形式和方法对母猪所选生殖指标的影响原文（非英语）冻精包装形式和方法对母猪所选生殖指标的影响[波兰语]

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The aim of this work was to estimate the influence of packaging forms and freezing methods of the semen of boars on the reporductive performance of sows. Experiments were carried out with 50 sows in a large swine farm. The females in experimental groups were inseminated with FT whilst in the control groups with liquid semen. Ejaculates from 4 boars were frozen in a polystyrene box onto static LN nitrogen vapor in 5 ml maxi and 0.5 ml medium straws according to methods A and B, modifications of the Westendorf et al (1975) and of the Pursel and Par (1987) technique, respectively. The sows were divided into five research groups, ten pigs in each group. The sows in experimental groups 1, 2, 3 and 4 were inseminated with frozen semen whilst in control group 5 with liquid semen. The females in groups 1 and 2 were inseminated with the semen frozen using method A and confectioned in a 0.5 ml medium and in 5 ml maxi straws, respectively. Whilst the sows in the group 3 and 4 were inseminated with the semen frozen according to method B and packaged in 0.5 ml and 5 ml straws, respectively, in each group. One insemination dose consisted of either 5 billion spermatozoa placed into one 5 ml maxi straw or of ten 0.5 ml medium starws with 500 million spermatozoa. The sows were inseminated twice or three times every ten hours with frozen semen. The first insemination took place 24 h cervically, three times every 10 h during each oestrus. One insemination dose comprises 5 x 10(9) of sperm and 5mg of PGF, alpha. The occurrence of ovulation was detected by ultrasonography. Efficiency of insemination and reproductive performance of sows have been evaluated on the basis of conception and farrowing rates and total piglets born in a litter. The conception rate, the farrowing rate and the total piglets born in a litter in all five groups (1, 2, 3, 4, 5) were: 12.0 +/- 1.8, respectively. A total of 40 females were inseminated with frozen (experimental) and 10 with liquid semen. There were no statistically significant differences in pregnancy and farrowing rates between all the experimental groups. Sows inseminated with liquid semen gave significantly higher percentages of litter size than females inseminated with frozen semen (12.00 vs. 10.19) (Table 1). Sows in groups 1 and 3 inseminated with semen frozen in 0.5 ml straws according to methods A and B had significantly higher litter sizes than sows in groups 2 and 4 inseminated with semen frozen in 5 ml straws according to corresponding methds A and B (10.62 and 10.77 vs. 9.42 and 9.71), respectively (Table 2). Animals inseminated with semen frozen in 0.5 ml straws had significantly higher percentages of litter size than females inseminated with semen frozen in 0.5 ml straws (10.7 vs 9.57) (Table 3). There were no significant differences in litter size between females inseminated with semen frozen according to methods A and B (10.06 vs 10.31) (Table 4). Acceptable reproductive performance of female pigs after AI with frozen semen was probably achieved because special attention was paid to the heat detection and timing of insemination related to ovulation (with the aid of rectal and abdominal ultrasonography), post-cervical insemination, addition of PGF(2)alpha to each insemination dose, improvement of the freezing-thawing methods and good freezability of the sperm' donors. Both freezing methods are relatively simple, but method B is less time consuming in preparation than method A. Fertility results obtained with frozen-thawed boar semen in our experiments are quite satisfactory.Thes results indicate that uner good conditions (insemination strategy) frozen boar semen can give results that approach those obtained with fresh semen.
机译：这项工作的目的是评估公猪精液的包装形式和冷冻方法对母猪再生产性能的影响。在一个大型养猪场对50头母猪进行了实验。实验组中的女性用FT精液授精，而对照组中则使用液体精液授精。根据方法A和B，Westendorf等人（1975）和Pursel and Par（1987）技术的改进方法，将4只公猪的射精在聚苯乙烯箱中冷冻在5 ml maxi和0.5 ml中型吸管中的静态LN氮气中。 ， 分别。将母猪分为五个研究组，每组十只猪。实验组1、2、3和4中的母猪用冷冻精液授精，而对照组5中则用液体精液授精。用方法A冷冻的精液对第1组和第2组中的雌性进行授精，分别在0.5ml培养基和5ml超大吸管中进行糖化。将第3组和第4组的母猪用按照方法B冷冻的精液进行授精，每组分别包装在0.5 ml和5 ml的吸管中。一剂受精剂量包括将50亿精子放入1毫升5毫升最大吸管中，或由10枚0.5毫升中性淀粉与5亿精子组成。每十小时用冷冻精液对母猪进行两次或三次授精。第一次受精是在子宫颈发情24小时内进行的，每次发情期间每10小时进行3次。一剂受精剂量包括5 x 10（9）的精子和5mg的PGF，α。通过超声检查发现排卵的发生。母猪的受精效率和繁殖性能已根据受胎率，产仔率和产仔数进行了评估。在所有五个组（1、2、3、4、5）中，受胎率，产仔率和窝产仔猪总数分别为：12.0 +/- 1.8。总共有40名雌性用冷冻（实验）授精，另外10名有液体精液进行授精。在所有实验组之间，妊娠和分娩率没有统计学上的显着差异。用液态精液授精的母猪的产仔数百分比明显高于用冷冻精液授精的母猪（12.00比10.19）（表1）。根据方法A和B，用0.5 ml吸管冷冻的精液进行授精的第1组和第3组母猪，与根据相应的方法A和B用5 ml吸管冷冻的精液进行授精的第2组和第4组的母猪相比，产仔数显着增加（10.62和分别为10.77与9.42和9.71）（表2）。用0.5 ml吸管冷冻的精液进行授精的动物的产仔量百分比显着高于用0.5 ml吸管冷冻的精液进行授精的雌性（10.7对9.57）（表3）。根据方法A和B冷冻的精液授精的雌性产仔数没有显着差异（10.06比10.31）（表4）。人工授精冷冻精液后雌猪的生殖性能可能达到了可接受的水平，因为要特别注意与排卵有关的热检测和授精时间（借助直肠和腹部超声检查），子宫颈授精，添加PGF（ 2）每个受精剂量的α值，冻融方法的改进和精子供体的良好可冻结性。两种冷冻方法都相对简单，但是方法B的制备时间比方法A少。在我们的实验中，使用冷冻解冻的公猪精液获得的育性结果是令人满意的。结果表明，条件不佳（授精策略）的冷冻公猪精液可以提供接近新鲜精液的结果。

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