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首页> 外文期刊>Methods: A Companion to Methods in Enzymology >Measuring calcium dynamics in living cells with genetically encodable calcium indicators.
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Measuring calcium dynamics in living cells with genetically encodable calcium indicators.

机译：使用可遗传编码的钙指示剂测量活细胞中的钙动力学。

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摘要

Genetically encoded calcium indicators (GECIs) allow researchers to measure calcium dynamics in specific targeted locations within living cells. Such indicators enable dissection of the spatial and temporal control of calcium signaling processes. Here we review recent progress in the development of GECIs, highlighting which indicators are most appropriate for measuring calcium in specific organelles and localized domains in mammalian tissue culture cells. An overview of recent approaches that have been undertaken to ensure that the GECIs are minimally perturbed by the cellular environment is provided. Additionally, the procedures for introducing GECIs into mammalian cells, conducting calcium imaging experiments, and analyzing data are discussed. Because organelle-targeted indicators often pose an additional challenge, we underscore strategies for calibrating GECIs in these locations.
机译：基因编码的钙指示剂（GECI）使研究人员能够测量活细胞内特定目标位置的钙动力学。这样的指标使解剖钙信号过程的空间和时间控制成为可能。在这里，我们回顾了GECIs发展中的最新进展，着重指出了哪些指标最适合用于测量哺乳动物细胞培养细胞中特定细胞器和局部区域的钙。提供了已采取的确保GECI受蜂窝环境影响最小的最新方法的概述。此外，还讨论了将GECIs引入哺乳动物细胞，进行钙成像实验以及分析数据的程序。由于以细胞器为指标的指标通常会带来额外的挑战，因此我们强调了在这些位置校准GECI的策略。

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