Immortalization of human keratinocytes with human papilloma virus DNA

Pritinder Kaur; Christine Halbert

首页> 外文期刊>Methods in cell science: an official journal of the Society for In Vitro Biology >Immortalization of human keratinocytes with human papilloma virus DNA

【24h】

Immortalization of human keratinocytes with human papilloma virus DNA

机译：人乳头瘤病毒DNA使人角质形成细胞永生化

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Human keratinocytes, derived from the cervix or foreskin, can be immortalized with the HPV-I6 or HPV-18 E6 and E7 genes. Two methods of Introducing the viral oncogenes into keratinocytes i.e. calcium phosphate transfection and retroviral transduction,are described below, both of which have been optimized for human keratinocytes. While the calcium phosphate transfection method can be used in a normal tissue culture facility, transduction with a retroviral vector containing oncogenes, requires a containment facility and appropriate laboratory practice.

机译：来源于宫颈或包皮的人角质形成细胞可以用HPV-16.6或HPV-18 E6和E7基因永生。下文描述了将病毒癌基因导入角质形成细胞中的两种方法，即磷酸钙转染和逆转录病毒转导，这两种方法均已针对人角质形成细胞进行了优化。尽管磷酸钙转染方法可用于正常的组织培养设备，但含有致癌基因的逆转录病毒载体的转导需要封闭设备和适当的实验室操作。

著录项

来源
《Methods in cell science: an official journal of the Society for In Vitro Biology》 |1995年第2期|共7页
作者
Pritinder Kaur; Christine Halbert;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类人体解剖学;人体生理学;
关键词
HPV-16; Human keratinocytes; Immortalization; Oncogenes E6 amp; E7; Transfection;

机译：HPV-16;人类角质形成细胞;免疫;癌基因E6和E7;转染;

相似文献

外文文献
中文文献
专利

1. Immortalization of human keratinocytes with human papilloma virus DNA [J] . Pritinder Kaur, Christine Halbert Methods in cell science: an official journal of the Society for In Vitro Biology . 1995,第2期

机译：人乳头瘤病毒DNA使人角质形成细胞永生化
2. Tyrphostins that suppress the growth of human papilloma virus 16-immortalized human keratinocytes. [J] . Ben Bassat-H, Rosenbaum Mitrani-S, Hartzstark Z, The Journal of Pharmacology and Experimental Therapeutics: Official Publication of the American Society for Pharmacology and Experimental Therapeutics . 1999,第3期

机译：抑制人乳头瘤病毒16永生化人角质形成细胞生长的酪氨酸磷酸化抑制剂。
3. Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes [J] . Takeuchi M, Takeuchi K, Kohara A, In Vitro Cellular and Developmental Biology. Animal: Journal of the Tissues Culture Association . 2007,第3a4期

机译：人乳头瘤病毒E6，E7和hTERT基因永生化的人间充质干细胞的染色体不稳定
4. Human Papilloma Virus biosensor based on electrochemical impedance spectroscopy of DNA hybridization [C] . Karimizefreh A., Sasanpour P., Jokar E., Institute of Electrical and Electronics Engineers International Conference on Electronics and Nanotechnology . 2014

机译：基于DNA杂交电化学阻抗谱的人乳头瘤病毒生物传感器
5. Associations between herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), cytomegalovirus (CMV), Epstein Barr virus (EBV), human papilloma virus (HPV), Chlamydia trachomatis, Neisseria gonorrhoeae infections and preeclampsia. [D] . Rustveld, Luis Orlando. 2005

机译：1型和2型单纯疱疹病毒（HSV-1和HSV-2），巨细胞病毒（CMV），爱泼斯坦巴尔病毒（EBV），人乳头瘤病毒（HPV），沙眼衣原体，淋病奈瑟氏球菌感染和先兆子痫之间的关联。
6. Large-scale analysis of protein expression changes in human keratinocytes immortalized by human papilloma virus type 16 E6 and E7 oncogenes [O] . Mark A Merkley, Ellen Hildebrandt, Robert H Podolsky, 2009

机译：大规模分析人类乳头瘤病毒16型E6和E7致癌基因永生化的人角质形成细胞中蛋白质表达的变化
7. Large-scale analysis of protein expression changes in human keratinocytes immortalized by human papilloma virus type 16 E6 and E7 oncogenes [O] . Mark A Merkley, Ellen Hildebrandt, Robert H Podolsky, 2009

机译：大规模分析人类乳头瘤病毒16型E6和E7致癌基因永生化的人角质形成细胞中蛋白质表达的变化

Immortalization of human keratinocytes with human papilloma virus DNA

摘要

著录项

相似文献

相关主题

期刊订阅