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Detection of the impairment of CD80 expression on circulating monocytes in HIV-infected Thai children

机译:检测感染艾滋病毒的泰国儿童循环单核细胞CD80表达的损伤

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The mechanism of progressive anergic response in HIV-infected children has yet to be adequately described. One possibility is inappropriate delivery of an essential second signal for T-cell activation due to the inappropriate presentation of co-stimulatory molecules. To determine whether the ligand for the secondary signal is impaired in pediatric AIDS, we compared the level of CD80 expression by circulating monocytes in HIV-infected and -noninfected children (15 mild/asymptomatic, 13 symptomatic and 12 HIV seronegative children). By two-color flow cytometry analysis, there was no statistically significant difference in the percentage of monocytes expressing CD80 among the groups (i.e., 63.2+/-5.8, 60.9+/-12.7, 61.04+/-10.9 for uninfected children, mild-asymptomatic children and symptomatic children, respectively). However, both infected groups showed statistically significant lower levels of CD80 expression, with mean fluorescent intensities of 40.9+/-5.9 and 38.8+/-10.7 compared to 57.05+/-16.3 for the uninfected control group. Our data demonstrated a correlation between HIV infection and impairment of CD80 by circulating monocytes. Whether the impairment on CD80 expression contributes to destruction of the immunological network in HIV-infected children requires further investigation.
机译:感染艾滋病毒的儿童进行性无痛反应的机制尚未得到充分描述。一种可能性是由于共刺激分子的不适当表达而导致不适当的用于T细胞活化的第二信号传递。为了确定小儿AIDS中次要信号的配体是否受损,我们比较了HIV感染和未感染儿童(15例轻/无症状,13例有症状和12例HIV血清阴性的儿童)中循环单核细胞的CD80表达水平。通过双色流式细胞术分析,各组之间表达CD80的单核细胞百分比没有统计学上的显着差异(即,未感染儿童,轻度儿童为63.2 +/- 5.8、60.9 +/- 12.7、61.04 +/- 10.9。无症状儿童和有症状儿童)。然而,两个感染组均显示出CD80表达的统计学显着降低水平,平均荧光强度为40.9 +/- 5.9和38.8 +/- 10.7,而未感染对照组为57.05 +/- 16.3。我们的数据表明,HIV感染与循环单核细胞CD80损伤之间存在相关性。 CD80表达的受损是否有助于破坏HIV感染儿童的免疫网络,需要进一步研究。

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