...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Microbial Pathogenesis >Alternative to antibiotics against Pseudomonas aeruginosa: Effects of Glycyrrhiza glabra on membrane permeability and inhibition of efflux activity and biofilm formation in Pseudomonas aeruginosa and its in vitro time-kill activity
【24h】

Alternative to antibiotics against Pseudomonas aeruginosa: Effects of Glycyrrhiza glabra on membrane permeability and inhibition of efflux activity and biofilm formation in Pseudomonas aeruginosa and its in vitro time-kill activity

机译：替代铜绿假单胞菌的抗生素：甘草对铜绿假单胞菌的膜通透性，外排活性和生物膜形成的抑制及其体外时间杀灭活性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The multi-drug resistance offered by Pseudomonas aeruginosa to antibiotics can be attributed towards its propensity to develop biofilm, modification in cell membrane and to efflux antibacterial drugs. The present study explored the activity of Glycyrrhiza glabra and one of its pure compounds, glycyrrhizic acid against P. aeruginosa and their mechanism of action in terms of the effect on membrane permeability, efflux activity, and biofilm formation were determined. Minimum inhibitory concentrations were determined by using broth dilution technique. The minimum bactericidal concentrations were assessed on agar plate. The MIC of the extract and glycyrrhizic acid was found to be 200 and 100 mu g ml(-1), respectively. The MBC was found to be 800 and 400 mu g ml(-1) in the case of extract and glycyrrhizic acid, respectively. Time-dependent killing efficacy was also estimated. Flowcytometric analysis with staining methods was used to determine the effect of extract and glycyrrhizic acid at 2 x MIC on different physiological parameters and compared it with the standard (antibiotic). The growth of P. aeruginosa was significantly inhibited by extract and the pure compound. The herbal extract and the glycyrrhic acid were also found to effective in targeting the physiological parameters of the bacteria that involve cell membrane permeabilization, efflux activity, and biofilm formation. This study reports the antipseudomonal action of Glycyrrhiza glabra and one of its compound and provides insight into their mode of action. (C) 2016 Elsevier Ltd. All rights reserved.
机译：铜绿假单胞菌对抗生素提供的多药耐药性可归因于其发展生物膜，修饰细胞膜和释放抗菌药物的倾向。本研究探讨了甘草及其纯化合物之一的甘草酸对铜绿假单胞菌的活性，并从对膜通透性，外排活性和生物膜形成的影响方面确定了它们的作用机理。通过使用肉汤稀释技术确定最小抑制浓度。在琼脂平板上评估最小杀菌浓度。提取物和甘草酸的MIC分别为200和100μg ml（-1）。发现在提取物和甘草酸的情况下，MBC分别为800和400μg ml（-1）。还估计了时间依赖性杀灭效力。用染色法进行流式细胞术分析以确定2x MIC下提取物和甘草次酸对不同生理参数的影响，并将其与标准品（抗生素）进行比较。提取物和纯化合物可显着抑制铜绿假单胞菌的生长。还发现草药提取物和甘草酸可有效靶向细菌的生理参数，这些细菌涉及细胞膜通透性，外排活性和生物膜形成。这项研究报告了甘草及其化合物之一的抗假性鼻行为，并提供了对其作用方式的见解。 （C）2016 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号