...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Microchemical Journal: Devoted to the Application of Microtechniques in all Branches of Science >Simultaneous determination of glycine betaine and arsenobetaine in biological samples by HPLC/ICPMS/ESMS and the application to some marine and freshwater fish samples
【24h】

Simultaneous determination of glycine betaine and arsenobetaine in biological samples by HPLC/ICPMS/ESMS and the application to some marine and freshwater fish samples

机译：HPLC / ICPMS / ESMS同时测定生物样品中的甘氨酸甜菜碱和砷甜菜碱及其在某些海洋和淡水鱼样品中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe a HPLC/mass spectrometry method for the simultaneous determination of glycine betaine and arsenobetaine in biological samples. The sample preparation included a water/methanol extraction followed by clean-up of extracts on a strong cation-exchange resin; the HPLC system consisted of a cation-exchange column with an ammonium formate buffer solution as mobile phase. Glycine betaine and arsenobetaine were quantified in a single chromatographic run by splitting the HPLC flow with an adjustable flow splitter and detecting glycine betaine selectively by electrospray MS in the positive single ion monitoring mode at m/z 118, and arsenobetaine with the arsenic-selective detector ICPMS at m/z 75. The proposed method was validated for arsenobetaine by analysis of CRM Dorm-2, and for glycine betaine by spiking the CRM Dorm-2 with a defined amount of glycine betaine. Finally, the developed method was applied to determine glycine betaine/arsenobetaine ratios in single specimens of four species of marine fish and one species of freshwater fish. (C) 2015 Elsevier B.V. All rights reserved.
机译：我们描述了同时测定生物样品中甘氨酸甜菜碱和砷甜菜碱的HPLC /质谱法。样品制备包括水/甲醇萃取，然后在强阳离子交换树脂上对萃取物进行净化。 HPLC系统由阳离子交换柱和甲酸铵缓冲溶液作为流动相组成。甘氨酸甜菜碱和砷甜菜碱在单个色谱中进行定量，方法是使用可调分流器分流HPLC流量，并以电喷雾MS在m / z 118的正单离子监测模式下选择性检测甘氨酸甜菜碱，并使用砷选择性检测器对砷甜菜碱进行检测。 ICPMS，m / z75。通过对CRM Dorm-2的分析，对拟议方法进行了砷甜菜碱的验证，对CRM CRM Dorm-2进行了定量的甘氨酸甜菜碱加样，对甘氨酸甜菜碱进行了验证。最后，将所开发的方法用于确定四种海洋鱼类和一种淡水鱼类的单个标本中的甘氨酸甜菜碱/砷甜菜碱比例。 （C）2015 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号