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首页> 外文期刊>Microbial Pathogenesis >Histone-like protein H-NS regulates biofilm formation and virulence of Actinobacillus pleuropneumoniae
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Histone-like protein H-NS regulates biofilm formation and virulence of Actinobacillus pleuropneumoniae

机译：组蛋白样蛋白H-NS调节胸膜肺炎放线杆菌的生物膜形成和毒力

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摘要

Actinobacillus pleuropneumoniae is the causative agent of porcine contagious pleuropneumonia, a very important swine respiratory infectious disease causing great economic losses worldwide. The pathogenesis of this disease is still not completely understood. Biofilm formation contributes to full virulence in many Gram-negative bacterial pathogens. In the present study, two biofilm-producing mutants were identified from the transposon mutagenesis mutant pools of A. pleuropneumoniae strain 4074 of serovar 1 (a non-biofilm forming strain). Inverse PCR and sequencing analysis revealed that the hns gene encoding the histone-like nucleoid structuring protein (H-NS) was inactivated by the mini-Tn10 transposon in both mutant strains. Further analysis revealed that the virulence was attenuated in the mutant strains when their haemolytic activity and 50% lethal doses in mice were compared with the parental strain. Real-time RT-PCR analysis suggested that the down-regulation of the exotoxin genes in the hns mutants may partly contribute to the virulence attenuation. Our data indicate that H-NS plays important roles in regulating biofilm formation and virulence in A. pleuropneumoniae.
机译：胸膜肺炎放线杆菌是猪传染性胸膜肺炎的病原体，猪传染性胸膜肺炎是一种非常重要的猪呼吸道传染病，在世界范围内造成巨大的经济损失。这种疾病的发病机理仍未完全了解。生物膜的形成有助于在许多革兰氏阴性细菌病原体中产生完全的毒力。在本研究中，从血清型1的胸膜肺炎链球菌4074菌株（非生物膜形成菌株）的转座子诱变突变体库中鉴定了两个产生生物膜的突变体。反向PCR和测序分析表明，在两个突变体菌株中，mini-Tn10转座子使编码组蛋白样核糖结构蛋白（H-NS）的hns基因失活。进一步的分析表明，将突变株的溶血活性和50％致死剂量与亲本株进行比较时，突变株的毒力减弱。实时RT-PCR分析表明，hns突变体中外毒素基因的下调可能部分促进了毒力的减弱。我们的数据表明，H-NS在胸膜肺炎链球菌的生物膜形成和毒力调节中起着重要作用。

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