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首页> 外文期刊>Microscopy and microanalysis: The official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada >Mice spermatogonial stem cells transplantation induces macrophage migration into the seminiferous epithelium and lipid body formation: High-resolution light microscopy and ultrastructural studies
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Mice spermatogonial stem cells transplantation induces macrophage migration into the seminiferous epithelium and lipid body formation: High-resolution light microscopy and ultrastructural studies

机译：小鼠精原干细胞移植诱导巨噬细胞迁移到生精上皮和脂质体形成：高分辨率光学显微镜和超微结构研究

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摘要

Transplantation of spermatogonial stem cells (SSCs), the male germline stem cells, in experimental animal models has been successfully used to study mechanisms involved in SSC self-renewal and to restore fertility. However, there are still many challenges associated with understanding the recipient immune response for SSCs use in clinical therapies. Here, we have undertaken a detailed structural study of macrophages elicited by SSCs transplantation in mice using both high-resolution light microscopy (HRLM) and transmission electron microscopy (TEM). We demonstrate that SSCs transplantation elicits a rapid and potent recruitment of macrophages into the seminiferous epithelium (SE). Infiltrating macrophages were derived from differentiation of peritubular monocyte-like cells into typical activated macrophages, which actively migrate through the SE, accumulate in the tubule lumen, and direct phagocytosis of differentiating germ cells and spermatozoa. Quantitative TEM analyses revealed increased formation of lipid bodies (LBs), organelles recognized as intracellular platforms for synthesis of inflammatory mediators and key markers of macrophage activation, within both infiltrating macrophages and Sertoli cells. LBs significantly increased in number and size in parallel to the augmented macrophage migration during different times post-transplantation. Our findings suggest that LBs may be involved with immunomodulatory mechanisms regulating the seminiferous tubule niche after SSC transplantation.
机译：在实验动物模型中，精子干细胞（雄性生殖干细胞）的移植已成功用于研究参与SSC自我更新和恢复生育能力的机制。然而，与理解用于临床疗法中的SSCs的受体免疫应答有关的还有许多挑战。在这里，我们已经使用高分辨率的光学显微镜（HRLM）和透射电子显微镜（TEM）对小鼠SSCs移植引起的巨噬细胞进行了详细的结构研究。我们证明，SSCs移植引发了快速有效的巨噬细胞募集到生精上皮（SE）。浸润性巨噬细胞源于将肾小管周围单核细胞样细胞分化为典型的活化巨噬细胞，它们活跃地通过SE迁移，积聚在小管腔中，并直接吞噬分化的生殖细胞和精子。 TEM定量分析显示，在浸润性巨噬细胞和支持细胞内，脂质体（LBs）的形成增加，脂质体被认为是细胞内炎性介质合成的平台和巨噬细胞激活的关键标志物。在移植后的不同时间，LBs的数量和大小显着增加，与巨噬细胞迁移的增加平行。我们的研究结果表明，LBs可能与SSC移植后调节生精小管生境的免疫调节机制有关。

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