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首页> 外文期刊>Microscopy research and technique >Tannase-mediated biotransformation assisted separation and purification of theaflavin and epigallocatechin by high speed counter current chromatography and preparative high performance liquid chromatography: A comparative study
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Tannase-mediated biotransformation assisted separation and purification of theaflavin and epigallocatechin by high speed counter current chromatography and preparative high performance liquid chromatography: A comparative study

机译：鞣酸酶介导的生物转化通过高速逆流色谱和制备型高效液相色谱辅助分离纯化茶黄素和表没食子儿茶素：对比研究

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摘要

A large scale isolation and purification of theaflavin (TF) and epigallocatechin (EGC) has been successfully developed by tannase-mediated biotransformation combining high-speed countercurrent chromatography. After tannase hydrolysis of a commercially available theaflavins extract (TE), the content of TF and EGC in tannase-mediated biotransformation product (TBP) achieved approximately 3 times enrichment. SEM studies revealed smooth tannase biotransformation and the possibility of recovery of the tannase. A single 1.5 hours' HSCCC separation for TF and EGC employing a two-phase solvent system could simultaneously produce 180.8 mg of 97.3% purity TF and 87.5 mg of 97.3% purity EGC. However, a preparative HPLC separation of maximum injection volume containing 120 mg TBP prepared 11.2 mg TF of 94.9% purity and 7.7 mg EGC of 89.9% purity. HSCCC separation demonstrated significant advantages over Prep HPLC in terms of sample loading size, separation time, environmental friendly solvent systems, and the production.
机译：通过鞣酸酶介导的生物转化结合高速逆流色谱法已成功开发了茶黄素（TF）和表没食子儿茶素（EGC）的大规模分离和纯化。鞣酸水解市售茶黄素提取物（TE）后，鞣酸酶介导的生物转化产物（TBP）中TF和EGC的含量约为3倍。 SEM研究表明，鞣酸酶具有平滑的生物转化作用，并且有恢复鞣酸酶的可能性。使用两相溶剂系统对TF和EGC进行1.5个小时的HSCCC分离，可以同时产生180.8 mg的97.3％纯度TF和87.5 mg的97.3％纯度EGC。但是，制备的最大进样量为120 mg TBP的制备型HPLC分离制备了纯度为94.9％的11.2 mg TF和纯度为89.9％的7.7 mg EGC。 HSCCC分离在样品上样量，分离时间，环境友好的溶剂系统和生产方面均显示出优于Prep HPLC的显着优势。

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