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Wnt signaling maintains the notochord fate for progenitor cells and supports the posterior extension of the notochord

机译：Wnt信号传递维持了祖细胞的脊索命运并支持脊索向后延伸

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摘要

The notochord develops from notochord progenitor cells (NPCs) and functions as a major signaling center to regulate trunk and tail development. NPCs are initially specified in the node by Wnt and Nodal signals at the gastrula stage. However, the underlying mechanism that maintains the NPCs throughout embryogenesis to contribute to the posterior extension of the notochord remains unclear. Here, we demonstrate that Wnt signaling in the NPCs is essential for posterior extension of the notochord. Genetic labeling revealed that the Noto-expressing cells in the ventral node contribute the NPCs that reside in the tail bud. Robust Wnt signaling in the NPCs was observed during posterior notochord extension. Genetic attenuation of the Wnt signal via notochord-specific beta-catenin gene ablation resulted in posterior truncation of the notochord. In the NPCs of such mutant embryos, the expression of notochord-specific genes was down-regulated, and an endodermal marker, E-cadherin, was observed. No significant alteration of cell proliferation or apoptosis of the NPCs was detected. Taken together, our data indicate that the NPCs are derived from Noto-positive node cells, and are not fully committed to a notochordal fate. Sustained Wnt signaling is required to maintain the NPCs' notochordal fate.
机译：脊索从脊索祖细胞（NPC）发展而来，并作为调节躯干和尾巴发育的主要信号中心。 NPC最初在gastrula阶段由Wnt和Nodal信号指定在节点中。然而，在整个胚胎发生过程中维持NPC促进脊索向后延伸的潜在机制仍不清楚。在这里，我们证明了NPC中的Wnt信号对于脊索的后延伸至关重要。遗传标记显示，腹侧结节中能表达诺托的细胞有助于驻留在尾芽中的NPC。在后胸索延长过程中观察到了NPC中强大的Wnt信号传导。 Wnot信号通过脊索特定的β-catenin基因消融的遗传衰减导致脊索的后截断。在这种突变体胚胎的NPC中，脊索特异性基因的表达被下调，并且观察到内胚层标记物E-钙粘着蛋白。未检测到NPC的细胞增殖或凋亡的显着改变。综上所述，我们的数据表明NPC来源于Noto阳性节点细胞，并没有完全致力于脊索动物的命运。需要持续的Wnt信号来维持NPC的脊索命运。

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