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Localization of RhoGEF2 during Drosophila cellularization is developmentally controlled by slam

机译：果蝇细胞化过程中RhoGEF2的定位受猛击控制。

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摘要

Essential for proper function of small GTPases of the Rho family, which control many aspects of cytoskeletal and membrane dynamics, is their temporal and spatial control by activating GDP exchange factors (GEFs) and deactivating GTPase-activating-proteins (GAPs). The regulatory mechanisms controlling these factors are not well understood, especially during development, when the organization and behaviour of cells change in a stage dependent manner. During Drosophila cellularization Rho signalling and RhoGEF2 are involved in furrow canal formation and the organization of actin and myosin. Here we analyze, how RhoGEF2 is localized at the sites of membrane invagination. We show that the PDZ domain is necessary for localization and function of RhoGEF2 and identify Slam as a factor that is necessary for RhoGEF2 localization. We also demonstrate that Slam can recruit RhoGEF2 to ectopic sites. Furthermore we find that the PDZ domain of RhoGEF2 can form a complex with Slam in vivo and that Slam transcripts and protein colocalize at the furrow canal and in basal particles. Based on these findings, we propose that accumulation of slam mRNA and protein at the presumptive invagination site provides a spatial and temporal trigger for RhoGEF2-Rho1 signalling
机译：通过控制GDP交换因子（GEF）和使GTPase激活蛋白（GAP）失活，可控制细胞骨架和膜动力学许多方面的Rho家族小GTP酶的正常功能至关重要。控制这些因素的调节机制还没有被很好地理解，特别是在发育过程中，当细胞的组织和行为以阶段依赖性方式改变时。果蝇细胞化过程中，Rho信号和RhoGEF2参与沟渠的形成以及肌动蛋白和肌球蛋白的组织。在这里，我们分析了RhoGEF2如何定位在膜内陷的位点。我们显示PDZ域对于RhoGEF2的定位和功能是必需的，并将Slam识别为RhoGEF2定位所必需的因素。我们还证明了Slam可以将RhoGEF2募集到异位部位。此外，我们发现RhoGEF2的PDZ结构域可以在体内与Slam形成复合物，并且Slam转录本和蛋白质在沟渠和基底颗粒中共定位。基于这些发现，我们建议在推测的内陷位点上灌满满贯mRNA和蛋白质，为RhoGEF2-Rho1信号传导提供时空触发

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