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首页> 外文期刊>Mechanisms of Development >Control of Cdc14 activity coordinates cell cycle and development in Caenorhabditis elegans.
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Control of Cdc14 activity coordinates cell cycle and development in Caenorhabditis elegans.

机译：Cdc14活性的控制协调了秀丽隐杆线虫的细胞周期和发育。

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Much of our understanding of the function and regulation of the Cdc14 family of dual-specificity phosphatases originates from studies in yeasts. In these unicellular organisms Cdc14 is an important regulator of M-phase events. In contrast, the Caenorhabditis elegans homolog, cdc-14, is not necessary for mitosis, rather it is crucial for G1/S regulation to establish developmental cell-cycle quiescence. Despite the importance of integrating cdc-14 regulation with development, the mechanisms by which this coordination occurs are largely unknown. Here, we demonstrate that several processes conspire to focus the activity of cdc-14. First, the cdc-14 locus can produce at least six protein variants through alternative splicing. We find that a single form, CDC-14C, is the key variant acting during vulva development. Second, CDC-14C expression is limited to a subset of cells, including vulva precursors, through post-transcriptional regulation. Lastly, the CDC-14C subcellular location, and thus its potential interactions with other regulatory proteins, is regulated by nucleocytoplasmic shuttling. We find that the active export of CDC-14C from the nucleus during interphase is dependent on members of the Cyclin D and Crm1 families. We propose that these mechanisms collaborate to restrict the activity of cdc-14 as central components of an evolutionarily conserved regulatory network to coordinate cell-cycle progression with development.Digital Object Identifier http://dx.doi.org/10.1016/j.mod.2011.06.001
机译：我们对Cdc14双特异性磷酸酶家族的功能和调控的许多了解都源于酵母的研究。在这些单细胞生物中，Cdc14是M期事件的重要调节剂。相比之下，秀丽隐杆线虫同源物 cdc-14 对于有丝分裂不是必需的，而对于G 1 / S的调节至关重要建立发育细胞周期的静止状态。尽管将 cdc-14 法规与发展相结合很重要，但这种协调发生的机制在很大程度上尚不清楚。在这里，我们证明了几个过程合谋集中了 cdc-14 的活动。首先， cdc-14 基因座可以通过选择性剪接产生至少六个蛋白质变体。我们发现，单一形式CDC-14C是外阴发育过程中的关键变异形式。其次，通过转录后调控，CDC-14C的表达仅限于细胞的一部分，包括外阴前体。最后，CDC-14C亚细胞的位置，以及因此与其他调节蛋白的潜在相互作用，是由核质穿梭调节的。我们发现，在相间期CDC-14C从细胞核的活跃出口取决于Cyclin D和Crm1家族的成员。我们建议这些机制协作以限制 cdc-14 的活动，因为它是进化上保守的调节网络的核心组成部分，以协调细胞周期与发育的进程。数字对象标识符http：//dx.doi。 org / 10.1016 / j.mod.2011.06.001

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