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首页> 外文期刊>Molecular & cellular proteomics: MCP >Immunoproteomic identification of human T cell antigens of Mycobacterium tuberculosis that differentiate healthy contacts from tuberculosis patients.
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Immunoproteomic identification of human T cell antigens of Mycobacterium tuberculosis that differentiate healthy contacts from tuberculosis patients.

机译：对结核分枝杆菌的人类T细胞抗原进行免疫免疫组化鉴定，可区分健康人和结核病患者。

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Identification of Mycobacterium tuberculosis antigens inducing cellular immune responses is required to improve the diagnosis of and vaccine development against tuberculosis. To identify the antigens of M. tuberculosis that differentiated between tuberculosis (TB) patients and healthy contacts based on T cell reactivity, the culture filtrate of in vitro grown M. tuberculosis was fractionated by two-dimensional liquid phase electrophoresis and tested for the ability to stimulate T cells in a whole blood assay. This approach separated the culture filtrate into 350 fractions with sufficient protein quantity (at least 200 microg of protein) for mass spectrometry and immunological analyses. High levels of interferon-gamma (IFN-gamma) secretion were induced by 105 fractions in healthy contacts compared with TB patients (p < 0.05). Most interesting was the identification of 10 fractions that specifically induced strong IFN-gamma production in the healthy contact population but not in TB patients. Other immunological measurements showed 42 fractions that induced significant lymphocyte proliferative responses in the healthy contact group compared with the TB patients. The tumor necrosis factor-alpha response for most of the fractions did not significantly differ in the tested groups, and the interleukin-4 response was below the detectable range for all fractions and both study groups. Proteomic characterization of the 105 fractions that induced a significant IFN-gamma response in the healthy contacts compared with the TB patients led to the identification of 59 proteins of which 24 represented potentially novel T cell antigens. Likewise, the protein identification in the 10 healthy contact-specific fractions vaccine or diagnostic targets.
机译：需要鉴定诱导细胞免疫应答的结核分枝杆菌抗原，以改善结核病的诊断和疫苗开发。为了基于T细胞反应性鉴定结核分枝杆菌的抗原，从而根据T细胞反应性区分结核分枝杆菌（TB）患者和健康接触者，通过二维液相电泳对体外生长的结核分枝杆菌的培养滤液进行分级分离，并测试其能力。在全血检测中刺激T细胞。这种方法将培养物滤液分离为350份级分，其中有足够的蛋白质量（至少200微克蛋白质）可用于质谱分析和免疫学分析。与结核病患者相比，健康接触中的105个组分诱导了高水平的干扰素-γ（IFN-γ）分泌（p <0.05）。最有趣的是鉴定在健康接触人群中特异性诱导强烈的IFN-γ产生的10个组分，但在结核病患者中却没有。其他免疫学测量结果显示，与结核病患者相比，健康接触组中有42个组分可诱导明显的淋巴细胞增殖反应。在测试组中，大多数组分的肿瘤坏死因子-α反应无显着差异，并且白细胞介素4反应低于所有组分和两个研究组的可检测范围。与结核病患者相比，在健康接触中诱导明显的IFN-γ反应的105个组分的蛋白质组学表征可鉴定出59种蛋白质，其中24种代表潜在的新型T细胞抗原。同样，在10种健康的接触特异性组分疫苗或诊断靶标中进行蛋白质鉴定。

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