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An improved PCR method for gender identification of eagles.

机译:一种改进的PCR方法,用于识别老鹰的性别。

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摘要

Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay.
机译:老鹰在性别上是单态的,因此很难确定其性别,这对于管理目的至关重要。在这项研究中,我们通过利用Spilornis cheela hoya的Chromo-Helicase-DNA结合蛋白(CHD)-Z和CHD-W基因之间的长度差异,开发了一种改进的性别识别方法。通过比较来自10种species类鹰的CHD-W和CHD-Z的DNA序列,我们设计了利用产品大小差异的通用性别鉴定PCR引物。已显示标准琼脂糖凝胶可轻松区分148-bp CHD-ZW和258-bp CHD-W PCR产物。当与28种雪松沙棘样品一起使用时,我们改良的通用引物可提供快速,精确的性别鉴定测定。

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