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首页> 外文期刊>Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines >Differentiation of Campylobacter jejuni and Campylobacter coli by polymerase chain reaction.
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Differentiation of Campylobacter jejuni and Campylobacter coli by polymerase chain reaction.

机译：空肠弯曲杆菌和大肠杆菌弯曲杆菌通过聚合酶链反应的区分。

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摘要

A multiplex PCR assay was developed using two primer sets for the identification and differentiation of Campylobacter coli and Campylobacter jejuni. Primer Set I amplifies a 460-bp fragment present in C. coli and C. jejuni. Set II amplifies a 160-bp target unique to C. jejuni. When the assay was performed on reference strains, amplification of C. coli yielded only the 460-bp fragment. Amplification of C. jejuni generated both the 160- and 460-bp fragments. Campylobacter field strains (n = 85) isolated from raw poultry were identified by PCR and by conventional biochemical methods. Species determination by the two methods agreed for 83 of the 85 isolates examined. By PCR, 23 were identified as C. coli and 62 as C. jejuni. One isolate was unidentifiable by biochemical testing. The PCR assay identified this isolated as C. coli. In addition, one strain which was identified as C. coli by biochemical testing was determined to be C. jejuni by PCR. The PCR assay offers an alternative to traditional biochemicaltyping methods for the identification and differentiation of C. coli and C. jejuni isolated from poultry. It is accurate, simple to perform, and can be completed within 8 h.
机译：使用两个引物组开发了多重PCR检测方法，用于鉴定和区分大肠弯曲菌和空肠弯曲菌。引物组I扩增存在于大肠杆菌和空肠弯曲杆菌中的460bp片段。组II扩增了空肠弯曲菌特有的160bp靶标。当对参照菌株进行测定时，大肠杆菌的扩增仅产生460bp的片段。空肠弯曲杆菌的扩增产生了160和460bp的片段。通过PCR和常规生化方法鉴定了从生禽中分离出的弯曲杆菌田间菌株（n = 85）。通过两种方法对85种分离株中的83种进行了物种测定。通过PCR，鉴定出23种为大肠杆菌，而62种为空肠弯曲菌。通过生化测试无法鉴定出一种分离物。 PCR测定法鉴定出该分离物为大肠杆菌。另外，通过生物化学测试鉴定为大肠杆菌的一种菌株通过PCR确定为空肠弯曲杆菌。 PCR测定法是传统生化分型方法的替代方法，用于鉴定和区分从家禽中分离的大肠杆状杆菌和空肠弯曲杆菌。它准确，易于执行，并且可以在8小时内完成。

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