• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Application of polymerase chain reaction to differentiate between strains of Campylobacter jejuni and Campylobacter coli
【24h】

Application of polymerase chain reaction to differentiate between strains of Campylobacter jejuni and Campylobacter coli

机译：聚合酶链反应在空肠弯曲菌和大肠弯曲菌之间的应用

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A polymerase chain reaction (PCR) assay was used to identify and differentiate between strains of?Campylobacter jejuni?and?Campylobacter coli. NineCampylobacter?reference strains;?C. jejuni?NCTC 11168,?C. jejuni?NCTC 11322,?C. jejuni?NCTC 11828,?Campylobacter coli?NCTC 12110,?C. coli?NCTC 11437,?C. coliNCTC 11350,?C. coli?NCTC 11366,?C. coli?NCTC 11438, and?C. coli?UA585were included in the assay. DNA primers derived from the genes VAC and SADC were used. PCR amplification of?C. coli?UA585 DNA with consensus sequence primers VAC1 and VAC2 resulted in a single DNA fragment of ~ 705 bp. The forward primer SADC1 and the reverse primer?SADC2?amplified a ~ 1750 bp product of?C. jejuniNCTC 11168 and?C. jejuni?NCTC 11828. ?A band of ~ 705 bp was also amplified from?C.?coli UA585 with the two pairs of primers SADC1 and SADC2. The results showed that oligonucleotides primers of VAC and SADC genes can be useful to identify?C. jejuni?and?C. coli.
机译：用聚合酶链反应（PCR）法鉴定和区分空肠弯曲杆菌和大肠弯曲杆菌的菌株。九株弯曲杆菌参考菌株; C。空空NCTC 11168，C.空肠NCTC 11322，C.空肠NCTC 11828，大肠杆菌弯曲杆菌NCTC 12110，C。大肠杆菌NCTC 11437，C。 coliNCTC 11350，℃。大肠杆菌NCTC 11366，C。大肠杆菌NCTC 11438和C。测定中包括大肠杆菌UA585。使用了来自基因VAC和SADC的DNA引物。 PCR扩增ΔC。带有共有序列引物VAC1和VAC2的大肠杆菌UA585 DNA产生约705 bp的单个DNA片段。正向引物SADC1和反向引物SADC22扩增出〜C的〜1750bp产物。 jejuniNCTC 11168和？空肠NCTC11828。用两对引物SADC1和SADC2从αC.UAUA585中扩增出约705bp的条带。结果表明，VAC和SADC基因的寡核苷酸引物可用于鉴定ΔC。空肠和？大肠杆菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号