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首页> 外文期刊>Molecular and Cellular Biochemistry: An International Journal for Chemical Biology >Overexpression of STAT3 in HPV-mediated cervical cancer in a north Indian population.
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Overexpression of STAT3 in HPV-mediated cervical cancer in a north Indian population.

机译：在印度北部人群中，HPV介导的子宫颈癌中STAT3的过度表达。

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The constitutively activated STAT family members, particularly STAT3, have been shown to possess transforming properties, and are strongly correlated with tumor development and progression. STAT3 transmits signals from many cytokines and growth factors to target genes in the nucleus through the Jak/Stat signaling pathway. HPV is the main etiological factor in the development of cervical cancer. In the current study, the expression of STAT3 was analyzed in various stages of HPV-mediated cervical carcinogenesis. Tissue biopsies from 100 patients with cervical cancer of different stages and normal tissues from patients undergoing hysterectomy were selected for studying the HPV status and STAT3 expression. HPV status of each corresponding biopsy was analyzed by PCR and typing. The mRNA expression was analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR). HPV infection was detected in majority of cases: 75% (9/12) in precancer, 85% (34/40) stage I & II, and 95% (36/38) in stage III & IV of cervical cancer cases by L1 PCR. Further sub typing revealed HPV16 in 100% (9/9) of L1 positives in precancerous & 90% (63/70) in different stages of cancer. Significant level of STAT3 mRNA expression was predominantly found in cervical cancer cases as compared to normal controls (P = 0.001). We also found a significant correlation of STAT3 expression in cases infected with HPV (P = 0.001). Our results indicate a potentially interactive effect between HPV 16/18 and transcriptional activation of STAT3 gene in cervical carcinogenesis. To our knowledge, this is the first such study to be reported from India. Further investigations are needed to determine the influence of STAT3 expression on cervical carcinogenesis and its possible interaction with HPV infection status.
机译：组成型活化的STAT家族成员，特别是STAT3，已被证明具有转化特性，并且与肿瘤的发生和发展密切相关。 STAT3通过Jak / Stat信号通路将来自许多细胞因子和生长因子的信号传输至细胞核中的靶基因。 HPV是宫颈癌发展的主要病因。在当前的研究中，分析了STAT3在HPV介导的宫颈癌发生的各个阶段的表达。选择100例不同时期宫颈癌患者的组织活检组织和子宫切除患者的正常组织作为研究HPV状态和STAT3表达的方法。通过PCR和分型分析每个相应活检的HPV状态。通过逆转录聚合酶链反应（RT-PCR）分析mRNA表达。在大多数病例中检测到HPV感染：通过L1，子宫颈癌的75％（9/12），I和II期的85％（34/40）和III和IV期宫颈癌的95％（36/38）。 PCR。进一步的亚型显示HPV16在癌前期的L1阳性中占100％（9/9），在癌症的不同阶段中占90％（63/70）。与正常对照组相比，主要在宫颈癌病例中发现了STAT3 mRNA表达的显着水平（P = 0.001）。我们还发现HPV感染病例中STAT3表达的显着相关性（P = 0.001）。我们的结果表明，HPV 16/18与STAT3基因的转录激活在宫颈癌发生过程中可能存在相互作用。据我们所知，这是印度首次报道的此类研究。需要进一步研究以确定STAT3表达对宫颈癌发生的影响及其与HPV感染状态的可能相互作用。

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