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首页> 外文期刊>Measurement Science & Technology >Two-color laser-induced fluorescent thermometry for microfluidic systems
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Two-color laser-induced fluorescent thermometry for microfluidic systems

机译：用于微流体系统的双色激光诱导荧光测温法

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摘要

The feasibility of implementing a two-color laser-induced fluorescence (LIF) technique to study thermal transport at the microscale is investigated. Temperature-sensitive (Rhodamine B) and temperature-insensitive (Sulforhodamine-101) fluorescent dyes are used in tandem to determine fluid temperature with high accuracy and low noise using a pulsed Nd:YAG laser as an illumination source. While the fluorescence intensity of the temperature-sensitive dye is proportional to temperature, it is also biased by variations in the illuminating intensity. Therefore, a second temperature-insensitive dye is required to compensate for such biases. Calibration of the two-color LIF system using the RhB-SR101 dye combination in ethanol and water yields temperature sensitivities of -1.5percent K~(-1) and -2.7percent K~(-1), respectively, with volumetric illumination from an Nd:YAG laser. The feasibility of this methodology for conducting temperature measurements is explored by measuring a steady-state temperature gradient maintained across a microfluidic channel array by two large hot and cold reservoirs. These measurements reveal that the mean steady-state temperatures in the microchannels are within +-0.4 deg C and +-0.3 deg C of the predicted temperatures with ethanol and water as the solvents, respectively, with a spatial resolution of 22.2 X 22.2 (mu)m. The experimental uncertainties in the measurements using the RhB-SR101 dye combination are +-0.48-0.59 deg C and +-0.41-0.49 deg C for ethanol and water, respectively.
机译：研究了实施双色激光诱导荧光（LIF）技术以研究微观尺度上的热传输的可行性。温度敏感（若丹明B）和温度不敏感（Sulforhodamine-101）荧光染料一起使用，以脉冲Nd：YAG激光作为照明源，以高精度和低噪声确定流体温度。尽管热敏染料的荧光强度与温度成正比，但它也会因照明强度的变化而产生偏差。因此，需要第二种对温度不敏感的染料来补偿这种偏差。使用RhB-SR101染料组合在乙醇和水中对双色LIF系统进行校准时，温度敏感度分别为-1.5％K〜（-1）和-2.7％K〜（-1），并通过Nd：YAG激光。通过测量两个大型冷热库在整个微流体通道阵列上保持的稳态温度梯度，探索了进行温度测量的这种方法的可行性。这些测量结果表明，在以乙醇和水为溶剂的情况下，微通道中的平均稳态温度分别在预测温度的+ -0.4摄氏度和+ -0.3摄氏度之内，空间分辨率为22.2 X 22.2（mu ）m。使用RhB-SR101染料组合进行测量时，实验不确定性分别是乙醇和水为+ -0.48-0.59℃和+ -0.41-0.49℃。

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