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首页> 外文期刊>Molecular biology of the cell >Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae
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Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae

机译：Cdc50p是一种保守的内体膜蛋白，可控制酿酒酵母中的极化生长

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摘要

During the cell cycle of the yeast Saccharomyces cerevisiae, the actin cytoskeleton and the growth of cell surface are polarized, mediating bud emergence, bud growth, and cytokinesis. We identified CDC50 as a multicopy suppressor of the myo3 myo5-360 temperature-sensitive mutant, which is defective in organization of cortical actin patches. The cdc50 null mutant showed cold-sensitive cell cycle arrest with a small bud as reported previously. Cortical actin patches and Myo5p, which are normally localized to polarization sites, were depolarized in the cdc50 mutant. Furthermore, actin cables disappeared, and Bni1p and Gic1p, effectors of the Cdc42p small GTPase, were mislocalized in the cdc50 mutant. As predicted by its amino acid sequence, Cdc50p appears to be a transmembrane protein because it was solubilized from the membranes by detergent treatment. Cdc50p colocalized with Vps21p in endosomal compartments and was also localized to the class E compartment in the vps27 mutant. The cdc50 mutant showed defects in a late stage of endocytosis but not in the internalization step. It showed, however, only modest defects in vacuolar protein sorting. Our results indicate that Cdc50p is a novel endosomal protein that regulates polarized cell growth. [References: 74]
机译：在酵母酿酒酵母的细胞周期中，肌动蛋白的细胞骨架和细胞表面的生长被极化，介导芽的出现，芽的生长和胞质分裂。我们确定CDC50为myo3 myo5-360温度敏感突变体的多拷贝抑制剂，该突变体在组织皮质肌动蛋白补丁方面存在缺陷。 cdc50 null突变体显示冷敏感的细胞周期停滞，并有小芽，如先前报道。通常在极化位点定位的皮质肌动蛋白补丁和Myo5p在cdc50突变体中被去极化。此外，肌动蛋白电缆消失了，Bdc1p和Gic1p，Cdc42p小GTP酶的效应子，在cdc50突变体中定位错误。正如其氨基酸序列所预测的那样，Cdc50p似乎是一种跨膜蛋白，因为它通过去污剂处理从膜中溶解了。 Cdc50p与Vps21p在内体区室共定位，并且也定位在vps27突变体中的E类区室。 cdc50突变体在胞吞后期显示出缺陷，但在内部化步骤中未显示出缺陷。然而，它显示了液泡蛋白分选中仅有中等缺陷。我们的结果表明，Cdc50p是一种新型的内体蛋白，可调节极化细胞的生长。 [参考：74]

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