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首页> 外文期刊>Molecular biology of the cell >Inducible systemic RNA silencing in Caenorhabditis elegans
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Inducible systemic RNA silencing in Caenorhabditis elegans

机译：秀丽隐杆线虫的诱导型系统性RNA沉默。

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摘要

Introduction of double-stranded RNA (dsRNA) can elicit a gene-specific RNA interference response in a variety of organisms and cell types. In many cases, this response has a systemic character in that silencing of gene expression is observed in cells distal from the site of dsRNA delivery. The molecular mechanisms underlying the mobile nature of RNA silencing are unknown. For example, although cellular entry of dsRNA is possible, cellular exit of dsRNA from normal animal cells has not been directly observed. We provide evidence that transgenic strains of Caenorhabditis elegans transcribing dsRNA from a tissue-specific promoter do not exhibit comprehensive systemic RNA interference phenotypes. In these same animals, modifications of environmental conditions can result in more robust systemic RNA silencing. Additionally, we find that genetic mutations can influence the systemic character of RNA silencing in C. elegans and can separate mechanisms underlying systemic RNA silencing into tissue-specific components. These data suggest that trafficking of RNA silencing signals in C. elegans is regulated by specific physiological and genetic factors. [References: 58]
机译：引入双链RNA（dsRNA）可以在多种生物和细胞类型中引发基因特异性RNA干扰反应。在许多情况下，这种反应具有系统性，因为在远离dsRNA传递位点的细胞中观察到基因表达的沉默。 RNA沉默的移动本质的分子机制尚不清楚。例如，尽管dsRNA的细胞进入是可能的，但是尚未直接观察到dsRNA从正常动物细胞的细胞离开。我们提供的证据表明，秀丽隐杆线虫的转基因菌株从组织特异性启动子转录dsRNA不会表现出全面的系统性RNA干扰表型。在这些相同的动物中，环境条件的改变可能导致更强健的全身RNA沉默。此外，我们发现遗传突变可以影响线虫中RNA沉默的系统特性，并且可以将系统性RNA沉默的基础机制分解为组织特异性成分。这些数据表明秀丽隐杆线虫中的RNA沉默信号的运输受特定的生理和遗传因素调控。 [参考：58]

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