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首页> 外文期刊>Molecular biology of the cell >The Chlamydomonas Fus1 protein is present on the mating type plus fusion organelle and required for a critical membrane adhesion event during fusion with minus gametes
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The Chlamydomonas Fus1 protein is present on the mating type plus fusion organelle and required for a critical membrane adhesion event during fusion with minus gametes

机译：衣原体Fus1蛋白存在于交配型和融合细胞器中，是与负配子融合过程中关键的膜粘附事件所必需的

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摘要

The molecular mechanisms of the defining event in fertilization, gamete fusion, remain poorly understood. The FUS1 gene in the unicellular, biflagellated green alga Chlamydomonas is one of the few sex-specific eukaryotic genes shown by genetic analysis to be essential for gamete fusion during fertilization. In Chlamydomonas, adhesion and fusion of the plasma membranes of activated mt+ and mt- gametes is accomplished via specialized fusion organelles. called mating structures. Herein, we identify the endogenous Fus1 protein, test the idea that Fus1 is at the site of fusion, and identify the step in fusion that requires Fus1. Our results show that Fus1 is a similar to95-kDa protein present on the external surface of both unactivated and activated mt+ gametes, Bioassays indicate that adhesion between mating type plus and mating type minus fusion organelles requires Fus1 and that Fus1 is functional only after gamete activation. Finally, immunofluorescence demonstrates that the Fus1 protein is present as an apical patch on unactivated gametes and redistributes during gamete activation over the entire surface of the microvillous-like activated plus mating structure, the fertilization tubule. Thus, Fus1 is present on mt+ gametes at the site of cell-cell fusion. and essential for an early step in the, fusion process. [References: 51]
机译：关于受精，配子融合的决定性事件的分子机制仍然知之甚少。单细胞，双鞭毛绿藻衣藻中的FUS1基因是少数几个具有性别特异性的真核基因之一，遗传分析表明这对于受精过程中的配子融合至关重要。在衣藻中，活化的mt +和mt-配子的质膜的粘附和融合是通过专门的融合细胞器完成的。称为交配结构。在本文中，我们确定了内源性Fus1蛋白，测试了Fus1在融合位点的想法，并确定了需要Fus1的融合步骤。我们的结果表明，Fus1与未激活和已激活的mt +配子外表面上存在的95 kDa蛋白相似，生物测定表明，交配型和交配型减去融合细胞器之间的粘附需要Fus1，而Fus1仅在配子激活后才起作用。最后，免疫荧光证明Fus1蛋白以顶端斑块的形式存在于未激活的配子上，并在配子激活期间在微绒毛状激活加交配结构（受精小管）的整个表面上重新分布。因此，Fus1存在于细胞与细胞融合部位的mt +配子上。对于融合过程的早期步骤至关重要。 [参考：51]

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