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首页> 外文期刊>Molecular biology reports >GhPSY, a phytoene synthase gene, is related to the red plant phenotype in upland cotton (Gossypium hirsutum L.)
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GhPSY, a phytoene synthase gene, is related to the red plant phenotype in upland cotton (Gossypium hirsutum L.)

机译：八氢番茄红素是一种八氢番茄红素合酶基因，与陆地棉的红色植物表型有关（陆地棉）。

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摘要

Carotenoids are important accessory pigments in plants that are essential for photosynthesis. Phytoene synthase (PSY), a rate-controlling enzyme in the carotenoid biosynthesis pathway, has been widely characterized in rice, maize, and sorghum, but at present there are no reports describing this enzyme in cotton. In this study, GhPSY was identified as a candidate gene for the red plant phenotype via a combined strategy using: (1) molecular marker data for loci closely linked to R1; (2) the whole-genome scaffold sequence from Gossypium raimondii; (3) gene expression patterns in cotton accessions expressing the red plant and green plant phenotypes; and (4) the significant correlation between a single nucleotide polymorphisms (SNP) in GhPSY and leaf phenotypes of progeny in the (Sub16 x T586) F-2 segregating population. GhPSY was relatively highly expressed in leaves, and the protein was localized to the plastid where it appeared to be mostly attached to the surface of thylakoid membranes. GhPSY mRNA was expressed at a significantly higher level in T586 and SL1-7-1 red plants than TM-1 and Hai7124 green plants. SNP analysis in the GhPSY locus showed co-segregation with the red and green plant phenotypes in the (Sub16 x T586) F-2 segregating population. A phylogenetic analysis showed that GhPSY belongs to the PSY2 subfamily, which is related to photosynthesis in photosynthetic tissues. Using a reverse genetics approach based on Tobacco rattle virus-induced gene silencing, we showed that the knockdown of GhPSY caused a highly uniform bleaching of the red color in newly-emerged leaves in both T586 and SL1-7-1 plants with a red plant phenotype. These findings indicate that GhPSY is important for engineering the carotenoid metabolic pathway in pigment production.
机译：类胡萝卜素是植物中重要的辅助色素，对光合作用至关重要。胡萝卜素生物合成途径中的速率控制酶植物苯丙酮合酶（PSY）已在水稻，玉米和高粱中得到广泛表征，但目前尚无报道在棉花中描述该酶。在这项研究中，通过组合策略使用以下方法将GhPSY鉴定为红色植物表型的候选基因：（1）与R1密切相关的基因座的分子标记数据; （2）雷蒙氏棉的全基因组支架序列; （3）棉花种质中表达红色和绿色植物表型的基因表达模式; （4）GhPSY中的单核苷酸多态性（SNP）与（Sub16 x T586）F-2分离种群中子代的叶子表型之间存在显着相关性。 GhPSY在叶子中相对高表达，并且该蛋白定位于质体，在该质体中它似乎主要附着在类囊体膜的表面。 GhPSY mRNA在T586和SL1-7-1红色植物中的表达水平明显高于TM-1和Hai7124绿色植物。在GhPSY基因座中的SNP分析显示，在（Sub16 x T586）F-2分离种群中，红色和绿色植物表型共分离。系统发育分析表明，GhPSY属于PSY2亚家族，与光合组织中的光合作用有关。使用基于烟草嘎嘎声病毒诱导的基因沉默的反向遗传学方法，我们证明了敲除GhPSY会导致T586和SL1-7-1植物与红色植物中新出现的叶片中红色高度均匀地脱色。表型。这些发现表明，GhPSY对于在色素生成中工程化类胡萝卜素代谢途径很重要。

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