Scale-Up of Suspension and Anchorage-Dependent Animal Cells

Bryan Griffiths

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Scale-Up of Suspension and Anchorage-Dependent Animal Cells

机译：悬浮和锚定依赖性动物细胞的规模扩大

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Alternative culture processes for laboratory scale-up (to 20 L) are described for both suspension and anchorage-dependent cells. Systmes range form simple multiple culture units such as the roller bottle, through stirred suspension and microcarrier unit bioreactors, to highly sophisticated perfusion culture capable of maintaining cells at densities of aobut 10~8/mL. Critical parameters in scale-up are discussed, and the advantages and disadvantages of each culture system are critically evaluated.

机译：描述了针对悬浮细胞和锚定依赖性细胞的用于实验室放大（至20 L）的替代培养过程。系统范围从简单的多个培养单元（例如滚瓶）到搅拌的悬浮液和微载体单元生物反应器，再到能够将细胞维持在约10〜8 / mL密度的高度复杂的灌注培养。讨论了扩大规模的关键参数，并严格评估了每种培养系统的优缺点。

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《Molecular biotechnology》 |2001年第3期|共14页
作者
Bryan Griffiths;
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正文语种 eng
中图分类分子生物学;生物工程学（生物技术）;
关键词
mammalian cell culture; suspension culture; mcirocarrier culture; culture scale-up; anchorage-dependent cells; animal cell bioreactors;

机译：哺乳动物细胞培养;悬浮培养;微载体培养;培养规模;锚定依赖性细胞;动物细胞生物反应器;

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1. Scale-Up of Suspension and Anchorage-Dependent Animal Cells [J] . Bryan Griffiths Molecular biotechnology . 2001,第3期

机译：悬浮和锚定依赖性动物细胞的规模扩大
2. Metabolic activity of anchorage-dependent animal cells at liquid/liquid interface in long-term culture [J] . Yoshiaki Shiba, Matabeei Katano, Takayuki Ohshima, 化学工学論文集 . 2001,第4期

机译：长期培养的液/液界面上锚定依赖性动物细胞的代谢活性
3. Metabolic activity of anchorage-dependent animal cells at liquid/liquid interface in long-term culture [J] . Yoshiaki Shiba, Matabeei Katano, Takayuki Ohshima, 化学工学論文集 . 2001,第4期

机译：长期培养中液/液界面处于锚固依赖性动物细胞的代谢活性
4. Cell attachment process in microcarrier culture for anchorage-dependent animal cells [C] . Naohiro Shiragami Asia-Pacific biochemical engineering conference;APBIOCHEC'97 . 1997

机译：微载体培养中锚定依赖性动物细胞的细胞附着过程
5. The effect of growth surface properties on the cultivation of anchorage-dependent animal cells and the development of novel surfaces for cell culture. [D] . Kent, Bradley Lane. 1995

机译：生长表面性质对锚定依赖性动物细胞的培养和细胞培养新表面的发展的影响。
6. Optimize Modulate and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor [O] . Carole Lambert, Julien Lemaire, Hélène Auger, 2019

机译：优化调节和按比例放大白藜芦醇和白藜芦醇二聚体在葡萄中的生物生产。从烧瓶到20 L生物反应器的细胞悬浮
7. Anchorage-dependent multicellular aggregate formation induces CD44 high cancer stem cell-like ATL cells in an NF-kappa B- and vimentin-dependent manner [O] . Miyatake, Yukiko, Sheehy, Noreen, Ikeshita, Shunji, 2015

机译：锚定依赖性多细胞聚集体形成以NF-κB和波形蛋白依赖性方式诱导CD44高癌干细胞样ATL细胞

Scale-Up of Suspension and Anchorage-Dependent Animal Cells

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