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首页> 外文期刊>Molecular biotechnology >Plant cell culture initiation. Practical tips.
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Plant cell culture initiation. Practical tips.

机译：植物细胞培养的启动。实用技巧。

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摘要

The use of cultured plant cells in either organized or unorganized form has increased vey considerably in the last 10-15 yr. Many new technologies have been developed and applications in both fundamental and applied research have led to the development of some powerful tools for improving our knowledge of botanical systems and for gaining external influence over some of the key processes involved in inter- and intracellular organization. This is particularly the case when cell culture techniques are combined with those for the genetic modification of plant cells. Being able to regenerate whole plants that have gained or lost the expression of one or more specific genes has revolutionized the way in which we approach scientific questions and has opened up many additional possibilities for the molecular dissection of plants. The success or fall of all plant cell culture technologies lies with culture initiation. The choice of plant material, its physiologival state and cultivation history, the media used, and their means of preparation are just some of the factors that can greatly influence whether the desired end result will be achieved. In this article are described some of the practical aspects involved in successful plant cell culture initiation and the choices that have to be made. Attention is given to some of the pitfalls that can occur and how to avoid them. A good start is half the work.
机译：在过去的10-15年中，以有组织或无组织形式使用培养的植物细胞已大大增加了产量。已经开发了许多新技术，并且在基础研究和应用研究中的应用都导致开发了一些强大的工具，这些工具可用于改善我们对植物系统的了解并在细胞间和细胞内组织涉及的某些关键过程中获得外部影响。当细胞培养技术与用于植物细胞遗传修饰的技术相结合时，尤其如此。能够再生获得或失去一种或多种特定基因表达的完整植物，彻底改变了我们处理科学问题的方式，并为植物分子解剖开辟了许多其他可能性。所有植物细胞培养技术的成败取决于培养的开始。植物材料的选择，其生理生长状态和栽培历史，使用的培养基及其制备方法只是可以极大地影响是否达到所需最终结果的一些因素。本文介绍了成功进行植物细胞培养起始的一些实际方面以及必须做出的选择。注意可能发生的一些陷阱以及如何避免这些陷阱。一个好的开始是成功的一半。

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