Differential display of mRNA.

Zhang JS; Duncan EL; Chang AC; Reddel RR

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Differential display of mRNA.

机译：mRNA的差异显示。

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Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as discrete bands following gel electrophoresis. This robust and relatively simple procedure allows identification of genes that are differentially expressed in different cell populations. Here we review DD including some recent modifications, and compare it with other techniques for analyzing differential mRNA expression.

机译：mRNA的差异显示（DD）是一种技术，其中细胞群表达的mRNA物种被逆转录，然后通过许多单独的聚合酶链反应（PCR）进行扩增。选择PCR引物和条件，以使任何给定的反应都能产生有限数量的扩增cDNA片段，从而使其在凝胶电泳后可视为离散的条带。这种鲁棒且相对简单的程序可以鉴定在不同细胞群体中差异表达的基因。在这里，我们审查DD，包括最近的一些修改，并将其与其他技术分析差异mRNA表达。

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《Molecular biotechnology》 |1998年第2期|共11页
作者
Zhang JS; Duncan EL; Chang AC; Reddel RR;
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正文语种 eng
中图分类分子生物学;生物工程学（生物技术）;
关键词
RNA; Messenger; oligo-(dT); DNA Primers; DNA; Complementary; Oligodeoxyribonucleotides; RNA; 信使;

机译：RNA;Messenger;oligo-(dT);DNA Primers;DNA;Complementary;Oligodeoxyribonucleotides;RNA;信使;

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专利

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机译：mRNA的差异显示。
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6. Differential subtraction chain a method for identifying differences in genomic DNA and mRNA. [O] . J H Luo, J A Puc, E D Slosberg, 1999

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Differential display of mRNA.

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