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首页> 外文期刊>Cancer prevention research. >Harnessing the Power of Cruciferous Vegetables: Developing a Biomarker for Brassica Vegetable Consumption Using Urinary 3,3 '-Diindolylmethane
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Harnessing the Power of Cruciferous Vegetables: Developing a Biomarker for Brassica Vegetable Consumption Using Urinary 3,3 '-Diindolylmethane

机译：利用十字花科蔬菜的力量：使用尿液3,3'-二吲哚甲烷开发甘蓝型油菜蔬菜消费的生物标志物

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Glucobrassicin in Brassica vegetables gives rise to indole-3-carbinol (I3C), a compound with potent anticancer effects in preclinical models. We previously showed that the urinary metabolite 3,3'-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of Brassica vegetables. However, the quantitative relationship between glucobrassicin exposure and urinary DIM level is unclear. We conducted a clinical trial to examine the hypotheses that a range of glucobrassicin exposure from Brassica vegetables is reflected in urinary DIM and that this effect plateaus. Forty-five subjects consumed vegetables, a mixture of brussels sprouts and/or cabbage, at one of seven discrete dose levels of glucobrassicin ranging from 25 to 500 mmol, once daily for 2 consecutive days. All urine was collected for 24 hours after each vegetable-eating session. Urinary DIM was measured using our published liquid chromatography-electrospray ionizationtandem mass spectrometry-selected reaction monitoring (LC/ESI-MS/MS-SRM) method. Urinary DIM excretion increased predictably with increasing glucobrassicin dose and plateaued between 200 and 300 mmol of glucobrassicin. The association between glucobrassicin dose and urinary DIM was strong and positive (R-2 = 0.68). The majority of DIM was excreted in the first 12 hours after vegetable consumption. We conclude that urinary DIM is a reliable biomarker of glucobrassicin exposure and I3C uptake and that feeding glucobrassicin beyond 200 mmol did not consistently lead to more urinary DIM, suggesting a plateau in potential chemopreventive benefit. (C) 2016 AACR.
机译：芸苔属蔬菜中的葡萄糖霉素会产生吲哚-3-甲醇（I3C），这是一种在临床前模型中具有有效抗癌作用的化合物。我们先前显示，尿代谢产物3,3'-二吲哚基甲烷（DIM）可以区分喂高剂量和低剂量芸苔属蔬菜的志愿者。但是，目前尚不清楚糖尿素暴露与尿中DIM水平之间的定量关系。我们进行了一项临床试验，以检验以下假设：尿DIM反映了芸苔属蔬菜中一定范围的葡糖溴素暴露，且这种影响达到平稳状态。四十五名受试者食用了蔬菜，布鲁塞尔芽甘蓝和/或卷心菜的混合物，其剂量为25至500 mmol的七个不连续剂量的葡糖糖素，连续2天每天一次。每次吃蔬菜后的24小时内收集所有尿液。尿中DIM的测定采用我们公开的液相色谱-电喷雾串联质谱-选择反应监测（LC / ESI-MS / MS-SRM）方法。尿DIM排泄随葡萄糖br素剂量的增加而增加，并在200至300 mmol的葡萄糖br素之间达到稳定。葡糖嘧啶的剂量与尿液DIM之间的相关性强且呈阳性（R-2 = 0.68）。食用蔬菜后的最初12小时内，大部分DIM会排出体外。我们得出的结论是，尿DIM是可靠的生物标志物，表明葡糖嘧啶暴露和I3C摄入，并且饲喂200毫摩尔以上的葡糖嘧啶并不能持续导致更多的尿DIM，这提示潜在的化学预防益处处于稳定状态。 （C）2016 AACR。

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